Proteins were measured using the proximal extension assay (PEA) technique [38 (link)] on a commercial proteomics array with 92 pre-selected proteins known or suggested to be involved in CVD (Olink Proseek Multiplex CVD I, Olink, Uppsala, Sweden). PEA is a homogeneous assay that utilizes pairs of antibodies equipped with DNA reporter molecules. When bound to their correct targets, the pairs give rise to new DNA amplicons that are subsequently quantified using a Fluidigm BioMark HD real-time PCR platform. Data are normalized and transformed using internal extension controls and inter-plate controls to adjust for intra- and inter-run variation [38 (link)]. A complete list of all proteins included in this study is found in Supplementary Materials Table S1 .
Proseek multiplex cvd 1
Manufactured by Olink
Sourced in Sweden
The Proseek Multiplex CVD I is a laboratory instrument designed for the analysis of cardiovascular disease (CVD) biomarkers. It utilizes a multiplex assay technology to simultaneously measure multiple protein targets in a single sample. The core function of the Proseek Multiplex CVD I is to quantify protein levels in biological samples, enabling researchers to investigate cardiovascular disease-associated molecular signatures.
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Lab products found in correlation
2 protocols using proseek multiplex cvd 1
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Proteomics Profiling of Cardiovascular Diseases
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Biomarker Measurement Protocol for CVD
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Measurement of plasma NT‐proBNP and hsTnT has been previously described.24 NT‐proBNP and hsTnT concentrations were determined by electro‐chemiluminescence immunoassay using the NT‐proBNP II and troponin T high‐sensitivity assays, respectively, on an ELECSYS Cobas e411 immunoanalyzer (Roche Diagnostics GmbH, Mannheim, Germany). The ranges of measurement for NT‐proBNP and hsTnT assays were 5 to 35 000 pg/mL and 3 to 10 000 pg/mL, respectively. Mean concentrations and interassay coefficient of variation of samples used for quality control, presented as mean (coefficient of variation), were established in‐house. Low‐concentration NT‐proBNP and hsTnT quality control samples averaged at 141 pg/mL (3.38%) and 26.7 pg/mL (6.66%), respectively; high NT‐proBNP and hsTnT samples averaged at 4759 pg/mL (4.03%) and 2090 pg/mL (4.06%), respectively.
A multiplex biomarker panel based on proximity extension technology was also utilized to get relative quantification on a panel of 92 biomarkers (Proseek Multiplex CVD I, Olink Proteomics AB, Uppsala, Sweden).
A multiplex biomarker panel based on proximity extension technology was also utilized to get relative quantification on a panel of 92 biomarkers (Proseek Multiplex CVD I, Olink Proteomics AB, Uppsala, Sweden).
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