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Triple resonance cryoprobe

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The Triple Resonance Cryoprobe is a specialized lab equipment designed for nuclear magnetic resonance (NMR) spectroscopy. It enables the detection and analysis of multiple nuclei within a sample simultaneously, providing enhanced sensitivity and resolution for complex molecular structure elucidation.

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Lab products found in correlation

Varian spectrometers, by Agilent Technologies (1 mentions) 900 mhz spectrometer, by Agilent Technologies (1 mentions) Deuterated solvent, by Cambridge Isotopes (1 mentions) Toluene, by Merck Group (1 mentions) Tlc silica gel 60 f254, by Merck Group (1 mentions) Microcal vp itc, by Malvern Panalytical (1 mentions) Inova 500 mhz, by Agilent Technologies (1 mentions)

Spelling variants of this product

Cryoprobe (12 citations) 5 mm HCN triple resonance cryoprobe (2 citations)

3 protocols using triple resonance cryoprobe

1

NMR Spectroscopy Protocol for Biomolecular Characterization

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All NMR experiments were recorded on 600 or 800 MHz Varian spectrometers equipped with a triple-resonance cryo-probe with pulse field gradient. All spectra were processed using NMRPipe67 (link) and analyzed using Sparky.68
Salmon L., Ahlstrom L.S., Horowitz S., Dickson A., Brooks CL I.I.I, & Bardwell J.C. (2016). Capturing a Dynamic Chaperone–Substrate Interaction Using NMR-Informed Molecular Modeling. Journal of the American Chemical Society, 138(31), 9826-9839.
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2

NMR Analysis of TLR5-HMGB1 Interactions

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drTLR5/HMGB1 interactions were determined by solution NMR spectroscopy on the CBP- and 6X His tags containing full-length and tailless 15N uniformly labeled HMGB1 proteins ranging from 80–100 µM, 10% (v/v) 2H20 in 10 mM Tris HCl (pH 8.0) and 1 mM deuterated DTT. Titrations of 1:1 and 1:2 (mol/mol) drTLR5/HMGB1 (CBP- and 6X His tag containing) were used All spectra were collected either in a Varian 800 MHz or 900 MHz spectrometer equipped with triple resonance cryoprobe at 25°C housed at Univ. Colorado Boulder and Denver campuses.
Das N., Dewan V., Grace P.M., Gunn R.J., Tamura R., Tzarum N., Watkins L.R., Wilson I.A, & Yin H. (2016). HMGB1 activates proinflammatory signaling via TLR5 leading to allodynia. Cell reports, 17(4), 1128-1140.
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3

Characterization of Organic Compounds

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Most chemicals were obtained from commercial sources and used as received. Solvents such as ethyl acetate, hexane, acetone, dichloromethane, were distilled before being used. Benzene, toluene, and tetrahydrofuran (THF) were used freshly distilled from metallic sodium. Deuterated solvents were obtained from Cambridge Isotope Laboratories Inc. Lithium hexamethyldisilylamide (LiHMDS) was used as a standard commercial solution in toluene from Sigma-Aldrich. Laboratories Flash chromatography was carried out on hand-packed silica gel 60 (230–400 mesh) columns. Merck TLC silica gel 60 F254 aluminum plates were used for thin-layer chromatography. NMR spectra were acquired using a Bruker Avance III 750 MHz (17.6 Tesla) equipped with a TCI triple resonance cryoprobe and in a Varian-Inova 500 MHz. Chemical shifts are reported in ppm (δ) and referenced to TMS (δH = 0 ppm). The spectral data required for the study comprise 1D (1H–NMR, 13C–NMR and titrations) and 2D (COSY, HSQC, HMBC, NOESY). Calorimetric data were obtained using a MicroCal VP-ITC.
Hernández-Lima J., Ramírez-Gualito K., Quiroz-García B., Silva-Portillo A.L., Carrillo-Nava E, & Cortés-Guzmán F. (2022). How solvent determines the molecular reactive conformation and the selectivity: Solvation spheres and energy. Frontiers in Chemistry, 10, 1012769.
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