Fluorometric kits

As previously described by Abu-Elfotuh et al. [41 (link)] briefly, brain monoamines (NE, DA, and 5 HT) were immediately detected fluorometrically, using the commercially available fluorometric kits (Sigma-Aldrich Co., St. Louis, MO, USA), according to the manufacturer’s instructions. Monoamines were detected by specific fluorescence at specific wavelengths of excitation and emission using Hitachi (F3010 model) spectrophotofluorometer [78 (link)]. The obtained fluorescence is read at wavelengths 320 nm and 480 nm for excitation and emission, respectively, for dopamine, wavelengths 380 nm and 480 nm for excitation and emission, respectively, for NE, and wavelengths 355 nm and 470 nm for excitation and emission, respectively, for serotonin.

The concentrations of brain monoamines, namely dopamine (DA), norepinephrine (NE), and serotonin (5-HT), were measured without delay using fluorometric kits that were commercially available (Sigma-Aldrich Co., St. Louis, MO, USA). As previously mentioned, the fluorometric method was employed to detect the monoamines present in the tissue samples, with specific excitation/emission wavelengths set at 320/480 nm for DA, 380/480 nm for NE, and 355/470 nm for 5-HT [62 (link)].