The immunoblotting was conducted as before [12] (link). The primary antibodies used were presented as follows, FLT3, p-AKT (Ser473), AKT, p-ERK (T202/Y204), Caspase-3, PARP, 4E-BP1 and p4E-BP1 (S65), which were all purchased from Cell Signaling Technology (Beverly, MA). ERK1/2, STAT5, p-STAT5 (Y694) and β-actin were obtained from Huabio (Hangzhou, China). p-FLT3 (Y591), MCL-1, BCL-XL, BCL-2, CDK-2, CDK-4 and CDK-6 were purchased from Abcam (Cambridge, MA).
Erk1 2
Manufactured by Huabio
Sourced in China
ERK1/2 is a lab equipment product designed for the detection and quantification of extracellular signal-regulated kinases 1 and 2 (ERK1/2). ERK1/2 are serine/threonine-specific protein kinases that are involved in the regulation of various cellular processes, including cell proliferation, differentiation, and survival.
Automatically generated - may contain errors
Lab products found in correlation
Bcl 2, by Abcam (1 mentions)
β actin, by Huabio (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Bcl xl, by Abcam (1 mentions)
Perk t202 y204, by Cell Signaling Technology (1 mentions)
P 4ebp1 s65, by Cell Signaling Technology (1 mentions)
4e bp1, by Cell Signaling Technology (1 mentions)
Phospho erk1 2, by Huabio (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Electrochemiluminescence reagent, by Merck Group (1 mentions)
β actin, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Selleck Chemicals (1 mentions)
β actin, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Histone h3, by Proteintech (1 mentions)
2 protocols using erk1 2
1
Immunoblotting Assay for Signaling Proteins
2
Western Blot Analysis of Key Signaling Proteins
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Cells were collected and digested in RIPA buffer in the presence of 1% protease inhibitor cocktail (Bimake). Proteins were separated by SDS‐PAGE and then transferred to PVDF membranes (Millipore, USA). After blocking in 5% skimmed milk, the membranes were incubated with antibodies to NSD3 (Cell Signaling Technology), β‐actin (Zhong Shan‐Golden Bridge), Histone‐H3 (proteintech), DiMethyl‐Histone H3 (ZENBIO), EGFR (ZENBIO), phospho‐EGFR (Tyr1173, ZENBIO), ERK1/2 (HUABIO), and phospho‐ERK1/2 (HUABIO) at 4°C overnight. The next day, blots were rinsed before application of a secondary antibody at room temperature for 90 min. Immunocomplexes were detected by electrochemiluminescence reagent (Millipore), with β‐actin as loading control.
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