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Phospholipid quantification assay kit

Manufactured by Merck Group
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The Phospholipid Quantification Assay Kit is a laboratory tool designed to measure the concentration of phospholipids in biological samples. The kit uses a colorimetric method to quantify the amount of phospholipids present.

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Lab products found in correlation

High sensitivity triglyceride fluorometric assay kit, by Merck Group (1 mentions) Triglyceride assay kit, by Abcam (1 mentions)

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Phospholipids (7 citations)

2 protocols using phospholipid quantification assay kit

1

Lipid Quantification in Cultured Cells

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Two million cells were plated in T75 flask and allowed to grow for 48 h. Then, 500,000 cells were plated in 6-well plates. After 24 h, cells from two wells were scraped in PBS and lipids extracted using the same protocol as for TLC (Bligh & Dyer protocol). After drying under nitrogen, lipids were solubilized in 200 μl of ethanol 95%. Quantifications were then performed according to the manufacturers’ instructions (Cholesterol Amplex Red from Thermo Fisher Scientific [A12216], High Sensitivity Triglyceride Fluorometric Assay Kit from Sigma-Aldrich [MAK264-1KT,] and Phospholipid quantification Assay Kit from Sigma-Aldrich [CS0001-1KT]).
Zouiouich M., Di Mattia T., Martinet A., Eichler J., Wendling C., Tomishige N., Grandgirard E., Fuggetta N., Fromental-Ramain C., Mizzon G., Dumesnil C., Carpentier M., Reina-San-Martin B., Mathelin C., Schwab Y., Thiam A.R., Kobayashi T., Drin G., Tomasetto C, & Alpy F. (2022). MOSPD2 is an endoplasmic reticulum–lipid droplet tether functioning in LD homeostasis. The Journal of Cell Biology, 221(6), e202110044.
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2

Quantifying Plasma Lipid Profiles

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Plasma triglycerides were measured using Triglyceride Assay Kit (Abcam, Cat no: ab65336) according to manufacturer’s instructions. Phospholipids were measured using Phospholipid Quantification Assay Kit (Sigma–Aldrich, Cat no: CS0001), according to manufacturer’s instructions. Assay fluorescence and absorbance was measured on a NOVOstar plate-reader, and concentrations calculated using a standard curve.
Walker M.E., De Matteis R., Perretti M, & Dalli J. (2024). Resolvin T4 enhances macrophage cholesterol efflux to reduce vascular disease. Nature Communications, 15, 975.
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