Anti atm 2c1

Manufactured by GeneTex

Sourced in United States

Anti-ATM (2C1) is a monoclonal antibody that targets the ATM (Ataxia Telangiectasia Mutated) protein. ATM is a serine/threonine protein kinase that plays a crucial role in the cellular response to DNA damage. The Anti-ATM (2C1) antibody can be used for the detection and analysis of ATM protein expression in various biological samples.

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Lab products found in correlation

Anti human lc3b nb100 2220, by Novus Biologicals (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti uhrf1, by Santa Cruz Biotechnology (1 mentions) Anti sod2 sc 30080, by Santa Cruz Biotechnology (1 mentions) Monoclonal anti tubulin e7, by Developmental Studies Hybridoma Bank (1 mentions)

2 protocols using anti atm 2c1

Antibody Panel for DNA Damage Response

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Anti-human LC3B (#NB100-2220) and anti-NBS1 (1D7, #GTX70224) were purchased from Novus Biologicals (Littleton, CO, USA). Antibodies for β-actin (#4970), pCHK1 (Ser-345, #2348), pATR (Thr-1989, #58014), pATM (Ser-1981, #13050), RAD50 (#3427), RPA (4E4, #2208), ULK1 (#8054S), PARP (#9542S), pCHK2 (Thr68, #2197) and cleaved-caspase-3 (#9664S) were from Cell Signaling Technology (Beverly, MA, USA). Anti-CHK1 (#SC-56291), anti-P21 (#SC-397), anti-ATR (#SC-515173), anti-ATRIP (#SC-365383) and anti-UHRF1 (#SC-373750) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-pRPA2 (S33) (#A300-246A) was from Bethyl Laboratories (Montgomery, TX, USA). Anti-pS824-KAP1 (#ab70369) was purchased from Abcam (Cambridge, UK). Horseradish peroxidase-conjugated (HRP) secondary antibodies and anti-ATM (2C1) (#GTX70103) were purchased from GeneTex Inc. (Irvine, CA, USA). Alexa Fluor® 488 goat anti-rabbit IgG (#A11008) were from Invitrogen/Thermo Fisher Scientific (Carlsbad, CA, USA).

Wang F., Jin S., Mayca Pozo F., Tian D., Tang X., Dai Y., Yao X., Tang J, & Zhang Y. (2021). Chemical screen identifies shikonin as a broad DNA damage response inhibitor that enhances chemotherapy through inhibiting ATM and ATR. Acta Pharmaceutica Sinica. B, 12(3), 1339-1350.

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Immunoblotting Analysis of Cellular Proteins

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SDS-PAGE and immunoblotting was performed using established protocols [41 (link)]. Nitrocellulose membranes were probed with anti-ATM (2C1, GeneTex, Irvine, CA), anti-RelA (sc-109, Santa Cruz Biotechnology, Santa Cruz, CA), anti-SOD2 (sc-30080, Santa Cruz Biotechnology) or monoclonal anti-tubulin (E7) obtained from the Developmental Studies Hybridoma Bank (Univ. of Iowa).

Dyer L.M., Kepple J.D., Ai L., Kim W.J., Stanton V., Reinhard M.K., Backman L.R., Streitfeld W.S., Babu N.R., Treiber N., Scharffetter-Kochanek K., McKinnon P.J, & Brown K.D. (2017). ATM is required for SOD2 Expression and Homeostasis within the Mammary Gland. Breast cancer research and treatment, 166(3), 725-741.

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