3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt and dimethyl sulfoxide dmso

Theabrownin powders (>90% of purity) and green tea crude extract were provided by Theabio Co., Ltd (Hangzhou, China; Batch number: 20151105001). Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), and 0.25% trypsin were obtained from Gibco BRL (Grand Island, NY, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were obtained from Sigma (St. Louis, MO, USA). Cell cycle kit was obtained from BD Biosciences (San Jose, CA, USA). All antibodies were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). Trizol reagent and real time polymerase chain reaction (real time PCR) kit were purchased from TaKaRa (Dalian, China). Transferase dUTP nick-end labeling (TUNEL) staining kit was purchased from Roche Applied Science (Indianapolis, IN, USA).

A. sativum stem was purchased from Nonghyup mart (Seoul, Korea) and stored in a refrigerator at −5 °C. Used in cell culture, Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and trypsin–EDTA were purchased from Gibco-BRL Co., Ltd. (Gaithersburg, MD, USA). We obtained 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Acetonitrile and acetic acid for HPLC grade were purchased from Thermo Fisher Sci., Inc. (Waltham, MA, USA). All other chemicals used in this experiment were Sigma-Aldrich’s analytical grade unless otherwise noted.