Av 3 hd spectrometer

Reagents and solvents for the synthesis of compounds were obtained from Sigma-Aldrich Corp. (Darmstadt, Germany). Organic solvents were further purified and/or dried using standard methods. Thin layer chromatography (TLC) was performed on Fluka silica gel (60 F254) plates (0.25 mm). Visualization was achieved using UV light at 254 nm, 10% sulfuric acid and ninhydrine. Column chromatography was performed on Merck silica gel 60 (size 70–230 mesh ASTM). Mass spectra (ESI-MS) were recorded on an Agilent 6410 MS instrument. ¹H and ¹³C NMR spectra of all precursors were recorded on a Bruker AV-III HD Bruker spectrometer at 400 MHz (¹H) and 100 MHz (¹³C). All NMR experiments were performed at 298 K. Chemical shifts were referenced with respect to tetramethylsilane (TMS). HRMS spectra of the final compounds (6a–c) were performed on an Agilent 6550 Series Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS Agilent 1290 Infinity II with Zorbax Eclipse Plus C18 (3.0 × 50 mm, 1.8 µm) column and gradient elution (solvent A: 0.1% HCOOH in water; solvent B: 0.1% HCOOH in acetonitrile).

Sulfamoyl chloride and compounds 1–3 (Supplementary Fig. 15) were prepared according to refs. ^{62 (link),63 (link)} with some changes detailed in Supplementary Methods. Reagents and solvents for the synthesis of the compounds were obtained from Sigma-Aldrich Corp. (Germany) and Bachem (Switzerland). Organic solvents were further purified and/or dried using standard methods. Thin layer chromatography (TLC) was performed on 0.25 mm TLC Silica gel 60 F₂₅₄ plates (Merck, cat: 105554). Visualization was achieved using UV light at 254 nm and ninhydrine. Column chromatography was performed on Silica gel 60 (size 70–230 mesh ASTM) column material (Millipore, cat: 107734). The ATR FT-IR spectrum was recorded on a FT-IR Perkin-Elmer Spectrum Two device (4000–400 cm⁻¹ region). Mass spectra (ESI-MS) were recorded on an Agilent Technologies 1200 series HPLC system. ¹H and ¹³C NMR spectra of all precursors were recorded on a AV-III HD Bruker spectrometer at 400 MHz (¹H) and 100 MHz (¹³C). All NMR experiments were performed at 298 K. Chemical shifts were referenced with respect to tetramethylsilane. The details about the MS and NMR protocols are given in Supplementary Methods.