We analyzed the gastric juice using a mass spectrometer API 4000Q TRAP (AB Sciex), First, we diluted the gastric juice 20- to 200-fold using distilled water. Then, diluted 100 µl of the gastric juice was mixed with an internal standard solution (CA-d5 ng/ml in 50% methanol). The mixed solution was then vortex-mixed for 3 sec, 200 µl of acetonitrile was added, and then centrifuged at 20,000 × g for 2 min. We injected 20 µl of the diluted supernatant that was diluted with 180 µl of 20 mM ammonium acetate. We used the standard component of Sigma-Aldrich C9377, G0759, C1129, T4009, L6250, D2510 for CA, CDCA, TCA, GCDCA, lithochholic acid (LCA), and deoxycholic acid (DCA) (Sigma-Aldrich Co. LLC). We analyzed LC-MS/MS data of each bile acid by the Analyst software version 1.6.3 (AB Sciex Pte. Ltd.).
L6250
Manufactured by Merck Group
Sourced in United States
The L6250 is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 6,000 rpm and a maximum relative centrifugal force (RCF) of 4,210 x g. The centrifuge can accommodate a variety of rotor types and sample volumes.
Automatically generated - may contain errors
Lab products found in correlation
C1129, by Merck Group (2 mentions)
T4009, by Merck Group (2 mentions)
Api 4000 qtrap, by AB Sciex (2 mentions)
Analyst software version 1, by AB Sciex (2 mentions)
Gcdca, by Merck Group (1 mentions)
Deoxycholic acid, by Merck Group (1 mentions)
Chenodeoxycholic acid, by Merck Group (1 mentions)
Taurocholic acid, by Merck Group (1 mentions)
Cholic acid, by Merck Group (1 mentions)
D2510, by Merck Group (1 mentions)
C9377, by Merck Group (1 mentions)
Lithocholic acid, by Merck Group (1 mentions)
2 protocols using l6250
1
Quantitative Analysis of Bile Acids in Gastric Juice
2
Quantification of Gastric Bile Acids
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The bile acid profiles of the aspirated gastric juice samples were measured using a mass spectrometer API 4000Q TRAP (AB Sciex, Redwood City, CA, U.S.A.). Initially, the gastric juice was diluted 20– 200-fold using distilled water. Then, 100 µL of the diluted gastric juice was mixed with an internal standard solution (CA-d5 ng/mL in 50% methanol). After mixing the solution by vortexing for 3 s, 200 µL acetonitrile was added, and the mixture was centrifuged at 20,000 × g for 2 min. Subsequently, 20 µL of the diluted supernatant, which was diluted with 180 µL of 20 mM ammonium acetate, was injected for analysis. Standard components of Sigma-Aldrich C9377, G0759, C1129, T4009, L6250, D2510 were used to measure the cholic acid (CA), chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glycochenodexoycholic acid (GCDCA), lithocholic acid (LCA), and deoxycholic acid (DCA), respectively (Sigma-Aldrich Co. LLC, St.Louis, USA). LC–MS/MS data for each bile acid were analyzed using Analyst software version 1.6.3 (AB Sciex Pte. Ltd. Redwood City, CA, USA).
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