After serum-free protein blocking (Dako Cytomation), cells were hybridized with various primary antibodies (overnight at 4°C), including goat polyclonal OPN (R&D systems), mouse anti-human residues 1–16, mAb clone 6E10 (1:100; Covance)], rabbit anti-Iba1 pAb (1:250; Wako Chemicals), rat anti-CD68 mAb (1:100; Abcam), rabbit anti-iNOS pAb (1:100; Cell signaling), goat anti-MMP9 pAb (1:100; R&D systems), Rat anti-CD36 mAb (1:xx; Abcam), rabbit anti-LC3 pAb (1:250; Novus), mouse anti-Golgi mAb (1:50; Sigma-Aldrich), rabbit anti-EEA1 pAb (1:100; Millipore), rabbit anti-Rab7 mAb (1:100; Abcam), rat anti-SCARA1 mAb (1:100; AbD Serotec), and anti IL-10 pAb (1:100; R&D system). Hybridization with primary antibodies was followed by incubation with appropriate secondary polyclonal antibodies (1h at 37°C; donkey anti-mouse, anti-rat, anti-goat, and anti-rabbit; 1:200; Jackson Immuno Research Laboratories) conjugated with Cy2, Cy3, and Cy5. Sections were mounted using ProLong Gold with DAPI (Molecular Probes, Life Technologies) and analyzed as previously described.
Goat anti mmp9 pab
Manufactured by R&D Systems
Goat anti-MMP9 pAb is a polyclonal antibody raised in goats against matrix metalloproteinase-9 (MMP9), also known as gelatinase B. The antibody is designed for the detection and quantification of MMP9 in various biological samples.
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Lab products found in correlation
2 protocols using goat anti mmp9 pab
1
Multimodal Immunofluorescence Profiling of Cells
2
Immunofluorescence Profiling of Alzheimer's Markers
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After serum-free protein block (Dako Cytomation) for 1 h at room temperature, cells were incubated with the following primary antibodies diluted in blocking solution for 1 h at 37°C: mouse anti-human amyloid-beta mAb clone 6E10 (1:100; Covance), rat anti-CD36 mAb (1:200; Abcam), rat anti-CD68 mAb (1:100; Abcam), rat anti-CD204 scavenger receptor type I/II (SCARA1) mAb (1:100; AbD Serotec), rabbit anti-EEA1 pAb (1:100; Millipore), rat anti-F4/80 mAb (1:100; Abcam), goat anti-MMP-9 pAb (1:100; R&D Systems), goat anti-TREM2 mAb (1:100; Abcam), and rabbit anti-IL-34 pAb (1:100; Biorbyt). Hybridization with primary antibodies was followed by incubation with appropriate secondary polyclonal antibodies (Cy2, Cy3, or Cy5 conjugated; 1:200; Jackson ImmunoResearch Laboratories) for 30 min at 37°C. The cells were then washed 3 times in PBS before being mounted using ProLong® Gold with DAPI (Molecular Probes, Life Technologies).
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