High capacity rna to cdna kit and sybr green master mix

Dulbecco's Modified Eagle Medium (DMEM), Roswell Park Memorial Institute Medium (RPMI-1640), Kaighn's Modification of Ham's F-12 Medium (F12K), penicillin-streptomycin (Invitrogen, Carlsbad, CA); Fetal bovine serum (FBS) (Atlanta Biologicals, Lawrenceville, GA); WST-1 proliferation assay kit (Roche, Indianapolis, IN); High-Capacity RNA-to-cDNA™ Kit and SYBR Green Master Mix (Applied Biosystems, Carlsbad, CA); Diff-Quick cell staining kit (Dade Behring, Inc., Newark, DE); In vitro Angiogenesis kit (EMD Millipore, Temecula, CA); anti-human IL-8 ELISA Kit (R&D Systems Inc., Minneapolis, MN); Gemcitabine (Sigma-Aldrich, St. Louis MO); Western blotting SuperSignal West Femto Maximum sensitivity substrate kit (Thermo Scientific, Logan, UT); goat anti-IL-8 antibody (Abcam, Cambridge, MA); biotinylated anti-β-actin (Sigma-Aldrich) and horseradish peroxidase (HRP) labelled secondary antibodies (1:2000; Santa Cruz Biotechnology).

The following reagents were used: Dulbecco’s Modified Eagle Medium (DMEM) (GE Healthcare Life Sciences, Logan, UT); Fetal Bovine Serum (FBS) (Atlanta Biologicals, Lawrenceville, GA); penicillin-streptomycin (Invitrogen, Carlsbad, CA); WST-1 proliferation assay kit (Roche, Indianapolis, IN); High-Capacity RNA-to-cDNA™ Kit and SYBR Green Master Mix (Applied Biosystems, Carlsbad, CA); Diff-Quick cell staining kit (Dade Behring, Inc., Newark, DE); anti-human IL-8 ELISA Kit (R&D Systems Inc., Minneapolis, MN); Gemcitabine (Sigma-Aldrich, St. Louis MO). Antibodies used were: anti-CD45, -CD68, -F4/80, -TGF-β1, -IL-8 (Abcam, Cambridge, MA), anti-arginase-1, anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) and anti-β-actin (Sigma-Aldrich). Western blotting SuperSignal West Femto Maximum sensitivity substrate kit was purchased from Thermo Scientific (Logan, UT). Immunohistochemical analysis reagent EZ-Dewax (Biogenex, Fremont, CA); background sniper, polymer, and probe were purchased from Biocare Medical (Concord, CA).