Fitc mouse anti human cd2

Immunofluorescence staining was performed as described previously (18 (link)). Briefly, primary antibodies were added to cells and incubated for approximately 90 minutes at 4 °C. Nuclei were stained with 4'6-diamidino-2-phenylindole (DAPI). All immunofluorescence staining results of in vitro cell culture were replicated more than 5 times. The primary antibodies used were FITC Mouse Anti-Human CD2 (BD Biosciences, 555326) and CD133/1 Antibody, anti-human, APC (Miltenyi Biotec, 130-113-668). All used antibodies were list in Table S1.

A single cell suspension was obtained by grinding and filtering tissue, and the A549 cell suspension was digested with trypsin. All cells were resuspended in wash buffer (PBS containing 0.1% BSA) and stained for surface markers using FITC Mouse Anti-Human CD2 (BD Biosciences, 555326), FITC Mouse Anti-Human CD4 (BD Biosciences, 550628), FITC Mouse IgG1, κ Isotype Control (BD Biosciences, 555748), CD133/1 Antibody, anti-human, APC (Miltenyi Biotec, 130-113-668), and isotype control antibodies, mouse IgG1, APC (Miltenyi Biotec, 130-113-196). FCM data were acquired with a FACSCalibur (BD Biosciences). All used antibodies were list in Table S1.