Paraffin sections of decidual implantation sites were heat-treated in 0.1M citrate buffer for 20min at 99.5°C. Slides were left in hot buffer for a further 20min for antigen unmasking. Anti-XCR1 (191704 from R&D Systems) was stained in Tris-buffered saline with 0.1% Tween for 45min. The staining was detected with goat anti-rabbit IgG-biotin and avidin-biotin-HRP complexes (Vector Laboratories).
Goat anti rabbit igg biotin and avidin biotin hrp complexes
Manufactured by Vector Laboratories
Goat anti-rabbit IgG-biotin and avidin-biotin-HRP complexes are laboratory reagents used in immunoassays and other applications. The goat anti-rabbit IgG-biotin component binds to rabbit immunoglobulin G (IgG), while the avidin-biotin-HRP complexes provide a means of signal detection and amplification.
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Lab products found in correlation
2 protocols using goat anti rabbit igg biotin and avidin biotin hrp complexes
1
Immunohistochemical Detection of XCR1
2
Decidual Implantation Site Immunostaining
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Paraffin sections of decidual implantation sites were heat treated in 0.1 M citrate buffer for 20 min at 99.5°C. Slides were left in hot buffer for a further 20 min for Ag unmasking. Anti-XCR1 (191704 from R&D Systems) was stained in TBS with 0.1% Tween 20 for 45 min. The staining was detected with goat anti-rabbit IgG-biotin and avidin-biotin-HRP complexes (Vector Laboratories).
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