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2 protocols using folin ciocalteu phenol reagent

1

Analytical Standards for Phenolic Compounds

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Gallic acid (GA), p-hydroxybenzoic
acid (pHBA), m-hydroxybenzoic acid
(mHBA), 3,4-dihydroxybenzoic acid (PCA, protocathecuic acid), vanillic
acid (VA), syringic acid (SyA), p-coumaric acid (CuA),
caffeic acid (CA), ferulic acid (FA), sinapic acid (SA), 5-caffeoylquinic
acid (CQA), quercetin (Q), kampferol (K), rutin (Ru), 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP), sodium azide, deuterium
oxide, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical were of analytical
grade, acquired from Sigma-Aldrich (Milano, Italy) and used as received.
The Folin-Ciocalteu phenol reagent was purchased from Sinopharm Chemical
Reagent (Shanghai, China). HPLC grade acetonitrile and methanol were
purchased from Carlo Erba (Milano, Italy); HPLC grade water was prepared
with the Milli-Q purification system (Millipore, Vimodrone, Italy).
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2

Extraction and Characterization of Rape Bran Polyphenols

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Commercial bread flour (Angel Yeast Co., Ltd., Yichang, China), instant yeast, sugar, salt, eggs, and butter for bread making were purchased from a local supermarket in Beijing. The dry rape BP was obtained from Sichuan Kuake Technology Development Co., Ltd. (Sichuan Province, China) without mildew and impurities. Sodium hydroxide, anhydrous ethanol, acetic acid, and petroleum ether were purchased from Beijing Chemical Industry Group Co., Ltd. (Beijing, China). Potassium ferrocyanide, ammonium molybdate, sodium acetate, sodium nitrite, and Folin-Ciocalteu phenol reagent were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Aluminium nitrate and gallic acid were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Rutin, α-amylase, neutral protease, glucoamylase, and diphenylpicrylhydrazyl (DPPH) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). All these chemicals were of analytical grade unless otherwise stated.
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