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Horseradish peroxidase hrp conjugated anti mouse antibody na931

Manufactured by GE Healthcare

Horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931) is a laboratory reagent used in various immunoassay techniques. It consists of a mouse antibody covalently linked to the enzyme horseradish peroxidase. This conjugate can be used to detect and quantify mouse antigens in samples.

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2 protocols using horseradish peroxidase hrp conjugated anti mouse antibody na931

1

Immunoblot and Immunofluorescence Analysis

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The following primary antibodies were used for immunoblot analysis: mouse antibody against α-actin (A2228) and rabbit antibody against HA (H6908) were both obtained from Sigma-Aldrich. Sheep antibody against HIV-1 p24 was purchased from Biochrom AG. Rabbit antibodies directed against viral Tat (ab43014) and SRSF2 (ab28428) were provided by Abcam. Mouse antibody against phosphorylated SR proteins (1H4G7) was obtained from Invitrogen. Rabbit antibody against MS2 was provided by Tetracore (TC7004). For detection, we used a horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931) from GE Healthcare, a HRP-conjugated anti-rabbit antibody (A6154) from Sigma-Aldrich and a HRP-conjugated anti-sheep antibody from Jackson Immunoresearch Laboratories Inc. For immunofluorescence analysis we used an anti-HA antibody (Sigma-Aldrich, clone HA-7) to detect the HA-tagged SRSF6 variants and an Alexa Fluor® 488 conjugated AffiniPure goat anti-mouse IgG (1:200; Jackson ImmunoResearch) as secondary antibody.
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2

Immunoblot Analysis of HIV-1 p24

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The following primary antibodies were used for immunoblot analysis: mouse antibody against α-actin (A2228) was obtained from Sigma-Aldrich. Sheep antibody against HIV-1 p24 was purchased from Biochrom AG. For detection, we used a horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931) from GE Healthcare and an HRP-conjugated anti-sheep antibody from Jackson Immunoresearch Laboratories Inc.
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