Ganetespib

Immunodeficient SCID mice were housed in a controlled, germ-free environment. Animals were randomized into treatment groups and anesthetized by isoflurane inhalation (2–3%, Forene) and HCT116 USP22^+/+ and USP22^−/− cells (1.0 × 10⁶ cells in Corning® Matrigel®) were injected subcutaneously into the left and right flank, respectively, using a 0.3 ml Micro-Fine syringe (BD Bioscience). The bodyweight, as well as the size of the growing tumors, were monitored daily. After the tumors reached a volume of approximately 100 mm³, 25 mg/kg Ganetespib (AdooQ) or vehicle solution (10% DMSO (v/v), 20% (v/v) pre-warmed Cremophor RH40 (55 °C; Sigma-Aldrich), 5% (w/v) dextrose) was injected i.v. for three consecutive days and after 4 days break, for 3 further days. Experiments were performed with six replicates per group in order to assure reproducibility. Experiments were performed blinded during tumor size measurement and subsequent immunohistochemical staining.