G1042

For Masson staining (G1006, Servicebio, Wuhan, China), dewaxing of tissue sections was carried out with xylene, downward gradient alcohol dehydration (100%, 95%, 80%, 70%), ascending gradient alcohol dehydration, 2.5% potassium dichromate overnight, immersion of Weigert iron hematoxylin and ponceau acid fuchsin, 1% phosphomolybdic acid and 2.5% aniline blue solution staining, ascending gradient alcohol dehydration (70%, 80%, 95%, 100%) and xylene transparency, and neutral gum seal piece. For Verhoeff’s Van Gieson (EVG) staining (G1035, Servicebio, Wuhan, China), dewaxing to water steps were as above, with Verhoeff’s Van Gieson differentiation after staining, Van Gieson counterstaining and alcohol dehydration, and sealing for observation. For Argyrophilic staining (G1042, Servicebio, Wuhan, China), dewaxing to water steps were as above, with ultrapure water washing after acid formaldehyde, silver glycine staining, 45-degree preheated reduction solution for color development, and microscope observation of dewatered sealing.

Five‐micrometre paraffin‐embedded sections of mouse abdominal aortic sections were used for haematoxylin–eosin (H&E, G1004&G1002, Servicebio, Wuhan, China) staining and Verhoeff‐Van Gieson (G1042, Servicebio) staining according to the manufacturer's instructions as previously reported.²⁹