Epi tech bisulfite

Manufactured by Qiagen

The Epi-Tech Bisulfite is a laboratory equipment designed for bisulfite conversion of DNA samples. The core function of this product is to perform the chemical conversion of unmethylated cytosine residues to uracil, while leaving methylated cytosine residues unchanged, as a critical step in the analysis of DNA methylation patterns.

Automatically generated - may contain errors

Lab products found in correlation

Pcr cloning kit, by Qiagen (3 mentions)

Spelling variants of this product

Epi Tech DNA bisulfite Kit (2 citations)

3 protocols using epi tech bisulfite

Genomic DNA Extraction and Bisulfite Conversion

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed with PBS, and genomic DNA was isolated using the Total DNA Extraction kit (iNtRON) according to the manufacturer’s protocol. Genomic DNA was treated with Epi-Tech Bisulfite (QIAGEN) according to the manufacturer’s recommendations, and used for PCR amplification. The PCR products were cloned using the PCR Cloning kit (QIAGEN).

Lee S.W., Wu G., Choi N.Y., Lee H.J., Bang J.S., Lee Y., Lee M., Ko K., Schöler H.R, & Ko K. (2018). Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells without Microenvironment of Feeder Cells. Molecules and Cells, 41(7), 631-638.

+ Open protocol

+ Expand

Genomic DNA Extraction and Bisulfite Conversion

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed with PBS, and genomic DNA was isolated using the Total DNA Extraction kit (Intron, Liberty Lake, WA, USA) according to the manufacturer’s protocol. Genomic DNA was treated with Epi-Tech Bisulfite (Qiagen) according to the manufacturer’s recommendations and used for PCR amplification. The PCR products were cloned using the PCR Cloning kit (Qiagen). Primer sequences are listed in Supplementary Table S4.

Lee Y., Lee S.W., Jeong D., Lee H.J., Choi N.Y., Bang J.S., Ham S, & Ko K. (2022). Inhibition of Class I Histone Deacetylase Enhances Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells. International Journal of Stem Cells, 16(1), 27-35.

+ Open protocol

+ Expand

Genomic DNA Bisulfite Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed with PBS, and genomic DNA was isolated using the Total DNA Extraction kit (iNtRON) according to the manufacturer’s protocol. Genomic DNA was treated with EpiTech Bisulfite (QIAGEN) according to the manufacturer’s recommendation, and used for PCR amplification. The PCR products were subcloned using the PCR Cloning kit (QIAGEN). Primers for bisulfite sequencing are listed in Supplementary Table 1.

Choi N.Y., Park Y.S., Ryu J.S., Lee H.J., Araúzo-Bravo M.J., Ko K., Han D.W., Schöler H.R, & Ko K. (2014). A Novel Feeder-Free Culture System for Expansion of Mouse Spermatogonial Stem Cells. Molecules and Cells, 37(6), 473-479.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!