Bicalutamide

MG132, DRB, cycloheximide, triptolide, and enzalutamide (ENZ) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). R1881 (methyltrienolone) was purchased from PerkinElmer, Inc. (Waltham, MA, USA). Bicalutamide was purchased from Thermo Fisher Scientific. Dihydrotestosterone (DHT), androstenedione (ASD), dehydroepiandrosterone (DHEA), flutamide, hydroxyflutamide (HO-Flut), and cyproterone acetate (CPA) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Testosterone, 5-bromo-2-deoxyuridine (BrdU), compound C (CC), bicalutamide, indinavir, insulin, 2-NBDG [2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxyglucose], Rhodamine/Phalloidin, RIPA and Dako mounting medium were from Thermo-Fisher Scientific (Rockford, IL, USA). Anti-β actin antibody (Monoclonal, mouse anti-rat) and PhosSTOP was purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). HK2 (Monoclonal, mouse anti-rat), anti-phospho-AMPK Thr 172 (Polyclonal, rabbit anti-rat) and anti-total AMPK (Polyclonal, rabbit anti-rat) antibodies were from Cell Signaling Technology (Danvers, MA, USA). The primers used in this study were synthesized and provide from Integrated DNA Technology (Danvers, MA, USA). Paraformaldehyde were from Electron Microscopy Sciences (Hatfield, PA, USA).