Phenylmethylsulfonyl fluoride pmsf

Manufactured by Bioworld Technology

Sourced in United States

Phenylmethylsulfonyl fluoride (PMSF) is a protease inhibitor commonly used in biochemical research. It functions by irreversibly inhibiting a wide range of serine proteases, thereby preventing the degradation of proteins during sample preparation and analysis.

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Lab products found in correlation

2 protocols using phenylmethylsulfonyl fluoride pmsf

Preparation of Cell Lysis Buffer

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Dimethyl sulfoxide (DMSO), ethylene glycol tetraacetic acid (EGTA), β-mercapto ethanol, CaCl₂, MgCl₂, NaOH, Triton X-100, ionomycin, NP-40, sodium deoxycholate, ammonium persulfate, KCl, formaldehyde, puromycin, penicillin, bromophenol blue, and ethanol were purchased from Sigma (USA). Phenylmethylsulfonyl fluoride (PMSF) was obtained from Bioworld (USA); and protease inhibitor cocktail, bovine serum albumin (BSA), and acrylamide solution were from GenDEPOT (USA). Sodium chloride (NaCl), ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and glycine were purchased from Duchefa (The Netherlands), and Tween 20 was obtained from Junsei Chemical Co., Ltd (Japan). Tris-HCl and glycerol were purchased from USB (USA).

Baek K.H., Yu H.V., Kim E., Na Y, & Kwon Y. (2016). Calcium influx-mediated translocation of m-calpain induces Ku80 cleavage and enhances the Ku80-related DNA repair pathway. Oncotarget, 7(21), 30831-30844.

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Purification and Characterization of Proteins

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Poly(acrylic) acid was obtained from Polysciences, Inc. (Warrington, PA).
Polyethylenimine (PEI) was purchased from Sigma (St. Louis, MO). Other
laboratory reagents were purchased from Fisher Scientific (Pittsburgh, PA, USA)
or Sigma. Bio-Rad Protein Assay reagents were purchased from Bio-Rad
Laboratories (Hercules, CA). Bovine serum albumin and 10 K molecular weight
cut-off (MWCO) ultrafiltration/diafiltration (UF/DF) devices were purchased from
Pierce (Rockford, IL). Kanamycin, leupeptin, and phenylmethylsulfonyl fluoride
(PMSF) were obtained from Bioworld (Dublin, OH). All sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE) products were purchased from
Invitrogen (Carlsbad, CA). Microcon centrifugal filter devices and ProSep-vA
High Capacity were purchased from Millipore (Bedford, MA). All antibodies and
HRP substrate were obtained from Bethyl Laboratories (Montgomery, TX).
Hydrophobic resins were purchased from GE Healthcare (Piscataway, NJ). MEP
HyperCel™ was purchased from Pall Life Sciences (Exton, PA). An AKTA
explorer with UNICORN software was used for all chromatography experiments, and
columns were purchased from GE Healthcare.

Fulton A., Lai H., Chen Q, & Zhang C. (2015). Purification of monoclonal antibody against Ebola GP1 protein expressed in Nicotiana benthamiana. Journal of chromatography. A, 1389, 128-132.

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