Live dead uv blue staining

Leupeptin, Pseudomonas exotoxin A, proteosomal inhibitors lactacystin and MG-132, monensin and brefeldin A were purchased from Sigma-Aldrich (Steinheim, Germany). Primaquine and Z-FL-COCHO (CSI) were obtained from Calbiochem (Darmstadt, Germany), bovine serum albumin was from Roth (Karlsruhe, Germany), saponin from Serva (Heidelberg, Germany) and butabindide oxalate was obtained from Tocris Bioscience (Bristol, UK). DQ-OVA, live-dead UV-blue staining and CFSE were obtained from Molecular Probes (Eugene, Oregon). Anti-mouse antibodies: CD3 (clone 500A2, V500 conjugated) and anti-IL-2 (clone JES6-5H4, APC-Cy7 conjugated) were obtained from BD Bioscience (USA); anti-CD4 (clone RM4-5, PE-Cy7 conjugated), anti-CD11c (clone N418, APC conjugated), anti-TNF-α (clone MPG-XT22, PerCP-eF710 conjugated), anti-CD107a (eBio1D4B, FITC conjugated), anti-NKp46 (29A1.4, eFluor660), anti-IL-4 (clone 11B11, APC conjugated), and anti-Thy1.1 (clone HIS51, Pe-Cy7 conjugated) were obtained from eBioscience Inc. (USA); and anti-CD8 (clone 53-6.7, BV650 conjugated), anti-CD11c (clone N418, PB conjugated), anti-IFN-γ (clone XMG1.2, BV711 and BV785 conjugated), LEAF™ Purified anti-mouse TNF-α, and mouse recombinant GM-CSF were obtained from Biolegend (USA).

BSA was purchased from Roth (Karlsruhe, Germany), saponin from Serva (Heidelberg, Germany), and live-dead UV-blue staining and CFSE were obtained from Molecular Probes (Thermo Fisher, Waltham, MA, USA). Anti-mouse antibodies: CD3 (clone 500A2, V500 conjugated) and anti-IL-2 (clone JES6-5H4, APC-Cy7 conjugated) were obtained from BD Bioscience (San Jose, CA, USA); anti-CD4 (clone RM4-5, PE-Cy7 conjugated), anti-TNF-α (clone MPG-XT22, PerCP-eF710 conjugated), anti-IL-4 (clone 11B11, APC conjugated) were purchased from eBioscience Inc. (eBioscience/Thermo Fisher, Waltham, MA, USA); and anti-CD8 (clone 53-6.7, BV650 conjugated), anti-IFN-γ (clone XMG1.2, BV711 and BV785 conjugated) were obtained from Biolegend (San Diego, CA, USA). IL-1β ELISA kit was purchased from Affymetrix (eBioscience/Thermo Fisher, Waltham, MA, USA). Multiple human cytokine detection kit, Human Th Cytokine Panel LEGENDplex was purchased from Biolegend (San Diego, CA, USA). R848 and CDA were purchased from InvivoGen (InvivoGen, Toulouse, France).