Cleancap cyanine 5 fluc mrna

Manufactured by TriLink

Sourced in United States, Germany

CleanCap® Cyanine 5 FLuc mRNA is a laboratory product produced by TriLink. It is a modified mRNA molecule that includes a Cyanine 5 fluorescent label and is designed for use in luciferase assays.

Automatically generated - may contain errors

Lab products found in correlation

Cleancap fluc mrna, by TriLink (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Cleancap egfp mrna, by TriLink (1 mentions) Cy5 mrna, by TriLink (1 mentions) Lysotracker green, by Thermo Fisher Scientific (1 mentions) Cleancap mcherry mrna, by TriLink (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions) Hela cell, by American Type Culture Collection (1 mentions) Quant it ribogreen reagent, by Thermo Fisher Scientific (1 mentions) Cleancap egfp mrna 5 mou, by TriLink (1 mentions) In vivo jetpei, by Polyplus Transfection (1 mentions)

3 protocols using cleancap cyanine 5 fluc mrna

Modified mRNA Molecules for Bioimaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Firefly luciferase mRNA (FLuc mRNA, 1929 nucleotides, CleanCap^® FLuc mRNA), FLuc mRNA modified with 5-methoxyuridine (FLuc mRNA (5moU), 1929 nucleotides, CleanCap^® FLuc mRNA (5moU)), enhanced green fluorescent protein mRNA (EGFP mRNA, 996 nucleotides, CleanCap^® EGFP mRNA), cyanine 5 (Cy5)-labeled FLuc mRNA modified with 5-methoxyuridine (Cy5-mRNA (5moU), CleanCap^® Cyanine 5 FLuc mRNA (5moU)), and ovalbumin mRNA modified with 5-methoxyuridine (OVA mRNA, 1437 nucleotides, CleanCap^® OVA (5moU) mRNA) were obtained from TriLink Biotechnologies (CA, USA). EZCap^TM Cyanine 5 FLuc mRNA (5moU) (Cy5-mRNA (5moU), 1921 nucleotides) was obtained from ApexBio Technology LLC (Boston, MA, USA).

Tang M., Sagawa A., Inoue N., Torii S., Tomita K, & Hattori Y. (2023). Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method. Pharmaceutics, 15(4), 1141.

+ Open protocol

+ Expand

mRNA Transfection and Luminescence Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

CleanCap Fluc mRNA, CleanCap mCherry mRNA, and CleanCap Cyanine 5 Fluc mRNA were purchased from TriLink Biotechnologies. QUANT-iT Ribogreen reagent, LysoTracker Green, and Hoechst 33342 were purchased from ThermoFisher Scientific. One-Glo + Tox were purchased from Promega. HEK293T cells and HeLa cells were purchased from ATCC.

Álvarez-Benedicto E., Farbiak L., Ramírez M.M., Wang X., Johnson L.T., Mian O., Guerrero E.D, & Siegwart D.J. (2022). Optimization of phospholipid chemistry for improved lipid nanoparticle (LNP) delivery of messenger RNA (mRNA). Biomaterials science, 10(2), 549-559.

+ Open protocol

+ Expand

Porcine Skin mRNA Delivery

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mRNAs (TriLink, San Diego, CA, USA) under investigation were administered to the viable porcine skin using various delivery systems. Two different mRNAs were used: (i) mRNA labeled with Cy5 (CleanCap™ Cyanine 5 FLuc mRNA (5 moU)—TriLink Biotechnologies, tebu-bio GmbH, Offenbach am Main, Germany) enabled visualization of the distribution of mRNA in the skin using widefield/confocal fluorescent microscopy and (ii) eGFP expressing mRNA (CleanCap™ eGFP mRNA (5 moU)—TriLink Biotechnologies, tebu-bio GmbH, Offenbach am Main, Germany), which reported on whether the mRNA delivered was expressed in the skin. Expression of eGFP post-administration of mRNA was visualized by fluorescence microscopy. Before administration, mRNAs were mixed with transfection agents. Two different transfection agents were studied; a polymer-based transfection agent (in vivo jet-PEI^®; Polyplus transfection, Illkirch, France) and a liposomal system (jetMESSENGER™; Polyplus transfection, Illkirch, France) made of ionizable mono-cationic lipids and co-helper phospholipids [35 (link)].

Darade A.R., Lapteva M., Hoffmann T., Mandler M., Schneeberger A, & Kalia Y.N. (2022). Effect of mRNA Delivery Modality and Formulation on Cutaneous mRNA Distribution and Downstream eGFP Expression. Pharmaceutics, 14(1), 151.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!