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Vasculife engs lifefactors kit

Manufactured by Lifeline Cell Technology
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Sourced in Germany

The VascuLife EnGS LifeFactors Kit is a cell culture media kit designed for the growth and maintenance of endothelial cells. The kit contains a proprietary formulation of growth factors, supplements, and other components required to support the in vitro culture of endothelial cells.

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Lab products found in correlation

Gentamicin, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by PromoCell (1 mentions) Amphotericin b, by Thermo Fisher Scientific (1 mentions) Has 2, by Merck Group (1 mentions) Atg5 sirna, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

2 protocols using vasculife engs lifefactors kit

1

Cultivation of Human Umbilical Vein Endothelial Cells

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HUVECs (passage 2–5) (Promocell, Heidelberg, Germany) were seeded in T75 cell culture flasks coated with 0.1% gelatin and cultivated at 37 °C with 5% CO2 in the Vasculife® EnGS EC culture medium (CellSystems, Troisdorf, Germany) containing the VascuLife EnGS LifeFactors Kit (Lifeline Cell Technology, Frederick, MD, USA), 50 mg/mL of gentamicin, and 0.05 mg/mL of amphotericin B (Thermo Fisher Scientific, Waltham, MA, USA). The medium was changed every 3 days. After reaching 80% confluency, the cells were detached using trypsin/EDTA (0.04%/0.03%, PromoCell, Heidelberg, Germany).
Weber J., Weber M., Feile A., Schlensak C, & Avci-Adali M. (2023). Development of an In Vitro Blood Vessel Model Using Autologous Endothelial Cells Generated from Footprint-Free hiPSCs to Analyze Interactions of the Endothelium with Blood Cell Components and Vascular Implants. Cells, 12(9), 1217.
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2

Culturing and Analyzing HUVEC Cells

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Human umbilical vein endothelial cells (HUVEC) were grown and maintained in basal media supplemented with VascuLife EnGS LifeFactors Kit (Lifeline Cell Technology, Oceanside, CA). Cells were plated on 0.2% (w/v) gelatin-coated cell culture plates (Thermo Fisher Scientific, Waltham, MA) and utilized within the first five passages. Antibodies for Western Blotting (WB) were purchased from the following sources and utilized at the designated dilutions: GAPDH (14C10, 1:10,000 dilution; Cell Signaling, Danvers, MA), LC3B (L7543, 1:1,000 dilution; Sigma), and HAS2 (sc-514737 and sc-365263 at 1:250 and 1:150, respectively; Santa Cruz Biotechnology, Dallas, TX). The following siRNA was purchased from Santa Cruz Biotechnology and used at the designated amounts: ATG5 siRNA (sc-41445, 100 pM) and scramble control siRNA-A (sc-37007, 100 pM).
Lüscher M., Hochstrasser U., Vogel G., Aeschbacher R., Galati V., Nelson C.J., Boller T, & Wiemken A. (2000). Cloning and Functional Analysis of Sucrose:Sucrose 1-Fructosyltransferase from Tall Fescue. Plant Physiology, 124(3), 1217-1228.
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