Protein samples (25–30 μg of total protein/lane) were mixed 1:1 with 2× protein loading buffer (60 mmol/L Tris‐HCl pH 6.8, 10% glycerol, 2% SDS, 1% bromophenol blue, 10% beta‐mercaptoethanol), boiled 5 min. at 98°C and loaded onto 12% SDS‐PAGE gels. Proteins were detected by Western blotting using: rabbit anti‐Cx43 antibody (C6219; Sigma‐Aldrich), mouse anti‐AIF antibody (MA5‐15880; Pierce‐Thermo Scientific), rabbit anti‐ETFB antibody (17925‐1‐AP; ProteinTech), mouse anti‐Na+/K+ ATPase alpha‐1 antibody (05‐369; Millipore, Billerica, MA, USA), mouse anti‐SDHA (MS204; MitoSciences, Eugene, OR, USA), mouse anti‐Pan Cadherin (C1821; Sigma‐Aldrich) and rabbit anti‐TOMM20 antibody (HPA011562; Sigma‐Aldrich).
Mouse anti pan cadherin
Manufactured by Merck Group
Sourced in United States
Mouse anti‐Pan Cadherin is a mouse monoclonal antibody that binds to the Pan Cadherin protein. Cadherins are a family of calcium-dependent cell-cell adhesion molecules that play a crucial role in cell-cell interactions and maintenance of tissue architecture. This antibody recognizes an epitope common to multiple cadherin family members.
Automatically generated - may contain errors
Lab products found in correlation
Hpa011562, by Merck Group (1 mentions)
Rabbit anti cx43 antibody, by Merck Group (1 mentions)
Mouse anti n cadherin, by Thermo Fisher Scientific (1 mentions)
Mouse anti e cadherin, by BD (1 mentions)
Rabbit anti β catenin, by Merck Group (1 mentions)
Mouse anti occludin, by Thermo Fisher Scientific (1 mentions)
Mouse anti ha, by Nacalai Tesque (1 mentions)
Rabbit anti claudin 1, by Thermo Fisher Scientific (1 mentions)
Mouse anti γ catenin, by BD (1 mentions)
Rabbit anti zo 1, by Thermo Fisher Scientific (1 mentions)
Dynabead, by Thermo Fisher Scientific (1 mentions)
Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse anti pan cadherin
1
Western Blot Protein Detection Protocol
2
Antibody Panel for Cell-Cell Adhesion
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The following antibodies were used: mouse anti-E-cadherin (Takara, Kyoto, Japan), anti-P-cadherin (Takara, Kyoto, Japan), mouse anti-desmoplakin (Progen, Heidelberg, Germany), mouse anti-desmocollin-2 (Invitrogen, Carlsbad, CA, USA), mouse antidesmoglein-2 (Progen), mouse anti-occludin (Invitrogen), mouse anti-pan-cadherin (Sigma), rabbit anti-claudin-1 (Invitrogen), rabbit anti-ZO-1 (Invitrogen), mouse anti-N-cadherin (Invitrogen), rabbit anti-β-catenin (Sigma-Aldrich, St. Louis, MO, USA), mouse anti-γ-catenin (BD Transduction Laboratories, Lexington. KY, USA), mouse anti-E-cadherin (BD Transduction Laboratories), and mouse anti-HA (Nacalai, Kyoto, Japan). The polyclonal anti-β-actin antibody was a gift from Dr. S. Imaoka. Lipofectamine LTX and Dynabeads were obtained from Invitrogen. Blebbistatin and 12-O-Tetradecanoylphorbol 13-acetate (TPA) were obtained from Calbiochem (San Diego, CA, USA).
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