Purified and polarized TH0, TH1, TH2, TH17 and iTreg cells were lysed with lysis buffer and cell homogenates were subjected to 10% SDS–PAGE (50 µg/lane) under reducing conditions. Gels were then electroblotted onto 0.45-µm nitrocellulose filters (Bio-Rad, Hercules, CA, USA) and were incubated with primary anti-GPR32 polyclonal mouse antibody (1:500, clone GTX71225, GeneTex), anti-ALX/FPR2 monoclonal rabbit antibody (1:500, clone FN-1D6-A1, Genovac) or with anti-β-actin monoclonal mouse antibody (1:10000, Bio-Rad), and then with secondary goat-anti-rabbit polyclonal antibody (1:2000, Santa Cruz Biotechnologies) for GPR32 and goat-anti-mouse polyclonal antibody (1:2000 for ALX and 1:10000 for β-actin).
Goat anti rabbit polyclonal antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Goat-anti-rabbit polyclonal antibody is an immunoglobulin product produced by immunizing goats with rabbit immunoglobulin. This antibody is designed to recognize and bind to rabbit antibodies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using goat anti rabbit polyclonal antibody
1
Western Blot Analysis of T Cell Subsets
2
Quantifying SIRT1 Expression in OSCC
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Immunohistochemical staining was performed to detect protein localization and expression in paraffin-embedded OSCC specimens. The sections were stained with rabbit anti-SIRT1 polyclonal antibody (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and goat anti-rabbit polyclonal antibody (1:5000; Santa Cruz Biotechnology), using an automatic slide stainer BenchMark XT (Ventana Medical Systems, Tucson, AZ, USA). Hematoxylin was used as the counterstain. Sections were evaluated using a microscope (Nikon, Tokyo, Japan) by one of the authors.
At higher magnification (×400), five visual fields were selected randomly, the expression positive signal was analyzed using Image-proplus software. SIRT1 protein levels in OSCC tissue and adjacent normal epithelium specimens were compared in accordance with the integral optical density (IOD) as a parameter for semiquantitative detection.
At higher magnification (×400), five visual fields were selected randomly, the expression positive signal was analyzed using Image-proplus software. SIRT1 protein levels in OSCC tissue and adjacent normal epithelium specimens were compared in accordance with the integral optical density (IOD) as a parameter for semiquantitative detection.
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