MMTV-PyMT;Apc+/+ and MMTV-PyMT;ApcMin/+ cells were isolated as previously described from primary mouse mammary tumors [11] and grown in RPMI 1640 media supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, and 1:5000 plasmocin (Invivogen). MMTV-PyMT;ApcMin/+ cells, expressing one truncated and one WT allele of APC, resemble the triple negative subtype of breast cancer cells (Supplemental Figure 1 ) [11] . Cells were passaged using 0.25% trypsin/EDTA and maintained at 37 °C with 5% CO2. Cells were plated and treated 24 hours later with each chemotherapeutic agent or solvent control: doxorubicin (500 nM, MP Biomedicals, LLC), etoposide (10 μM, Sigma), paclitaxel (2.5 μM, Sigma), cisplatin (16 μM, Sigma), VE-821 (10 μM, Selleckchem), KU-55955 (10 μM, Selleckchem), or NU-7441 (5 μM, Tocris). Drug treatments were performed for 24 hours unless otherwise indicated.
Ku 55955
Manufactured by Selleck Chemicals
KU-55955 is a chemical compound that functions as a selective and potent inhibitor of the serine/threonine protein kinase ATM (Ataxia Telangiectasia Mutated). It is commonly used in scientific research as a tool to study the role of ATM in various cellular processes.
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Lab products found in correlation
2 protocols using ku 55955
1
Comparative Analysis of MMTV-PyMT Breast Cancer Cells
2
Generating OXA-Resistant Gastric Cancer Cell Lines
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All mammalian cell lines used in this study were grown in a humidified 37 °C incubator with 5% CO2. Human gastric epithelial cell line GES1 and gastric cancer cell lines AGS and HGC27 were purchases from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). OXA-resistant gastric cancer cell lines, AGS-R and HGC27-R, were generated by subjecting cells to increasing concentrations of OXA (Ren et al., 2023 (link)). The process involved gradually exposing the cells to OXA at ascending concentrations ranging from 0.25 μg/mL to 6 μg/mL and 2 μg/mL, respectively. DNA repair inhibitors, namely, VE-821 (10 μM) and KU-55955 (10 μM), were procured from Selleckchem. Unless explicitly mentioned, the duration of drug treatment was set at 24 h.
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