To detect COL11A1 expression, IHC was performed on Ventana Discovery Ultra autostainer (Roche, Indianapolis, IN) with the following protocol and reagents. Vial of mAb anticol11A1 (Oncomatryx, High Concentration 2.3 mg/mL Rabbit monoclonal (Clone 1e8.33)). All reagents were provided by Roche, Indianapolis IN: Samples underwent heat-induced epitope retrieval, (Cell Conditioning Solution ULTRA CC1 (950-224, Roche)) for 32 min; the primary antibody was incubated for 30 min per manufacturers instruction; followed by DISCOVERY OmniMap anti-Rt HRP (760-4457; Roche), DISCOVERY ChromoMap DAB kit (760-159; Roche), Hematoxylin II (790-2208; Roche), and Bluing reagent (790-2037; Roche) were used for visualization. All samples (PDX, xenograft, primary, normal) were stained in along with positive control (LM7 xenograft) and negative control xenograft (143B COL11A1 KO xenograft), grown subcutaneously in NSG female mice, and tumors were harvested when they reached a size of 1000 mm3. Isotype controls were used as well.
Discovery omnimap anti rt hrp
Manufactured by Roche
The DISCOVERY OmniMap anti-Rt HRP is a laboratory equipment product developed by Roche. It is designed to detect and visualize specific targets in biological samples using horseradish peroxidase (HRP) conjugated secondary antibodies.
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2 protocols using discovery omnimap anti rt hrp
1
COL11A1 Expression Detection by IHC
2
Immunohistochemical Detection of COL11A1
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To detect COL11A1 expression, IHC was performed on Ventana Discovery Ultra autostainer (Roche, Indianapolis, IN) with the following protocol and reagents. Vial of mAb anticol11A1 (Oncomatryx, High Concentration 2.3 mg/mL Rabbit monoclonal (Clone 1e8.33)). All reagents were provided by Roche, Indianapolis IN: Samples underwent heat-induced epitope retrieval, (Cell Conditioning Solution ULTRA CC1 (950–224, Roche)) for 32 minutes; the primary antibody was incubated for 30 minutes per manufacturers instruction; followed by DISCOVERY OmniMap anti-Rt HRP (760–4457; Roche), DISCOVERY ChromoMap DAB kit (760–159; Roche), Hematoxylin II (790–2208; Roche), and Bluing reagent (790–2037; Roche) were used for visualization. All samples (PDX, xenograft, primary, normal) were stained in along with positive control (LM7 xenograft) and negative control xenograft (143B COL11A1 KO xenograft), grown subcutaneously in NSG female mice, and tumors were harvested when they reached a size of 1000 mm3. Isotype controls were used as well.
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