Quant it picogreen dsdna assay kit

Amplified viral DNA, cDNA and total microbial DNA were used for shotgun library construction. DNA concentration was measured by Quant-iT PicoGreen dsDNA Assay kit (Thermo Fisher Scientific), and the fluorescence was detected by EnVision Multilabel Plate Reader (Waltham, MA, USA). Libraries were made using an Illumina Nextera XT Samples Prep kit (Illumina, San Diego, CA, USA), quantified using both Quant-iT PicoGreen dsDNA Assay kit and KAPA Library Quantification kit (Kapa Biosystems, Basel, Switzerland). The size distribution of the libraries was checked by 5300 Fragment Analyzer (Agilent, Santa Clara, CA, USA). Libraries were pooled for sequencing. The concentration of the pooled libraries was measured using Qubit (Invitrogen, Carlsbad, CA, USA), and the size distribution of the pooled libraries was checked by Agilent Technology 2100 Bioanalyzer using a High Sensitivity DNA chip (Agilent). Sequence was acquired using the Illumina Miseq (250 bp paired-end reads, Illumina) and HiSeq (150 bp paired-end reads, Illumina).