Young mated female adults of 1) yw; mat αtub-Gal4[V37]/UASp-MoxMaple3-K10.TLS, 2) yw; mat αtub-Gal4[V37]/UASp-Msps-MoxMaple3-K10.TLS, and 3) yw; UASp-Klp10A-GFP-SspB/+; mat αtub-Gal4[V37]/UASp-Msps-MoxMaple3-K10.TLS were dissected in Halocarbon oil 700, as described above. Freshly dissected samples were imaged on a Nikon W1 spinning disk confocal microscope (Yokogawa CSU with pinhole size 50 µm) with a Hamamatsu ORCA-Fusion Digital CMOS Camera and a 40× 1.25 N.A. silicone oil lens. MoxMaple3 was photoconverted from green to red within a circle of ~12 µm diameter inside the oocytes by 405 nm light controlled by Mightex Polygon DMD Illuminator. The photoconverted signal was acquired at 0.5 µm/step in Z-stack images after photoconversion. Sum slices of the Z projection were used to calculate the nurse cell-to-oocyte ratio of the average intensity of the photoconverted signal.
Polygon dmd illuminator
Manufactured by Mightex
The Polygon DMD Illuminator is a specialized laboratory equipment designed to provide precise and controlled illumination for a variety of applications. It utilizes a digital micromirror device (DMD) to generate and project customizable light patterns onto a target area. The core function of the Polygon DMD Illuminator is to deliver dynamic and programmable illumination, enabling researchers and scientists to precisely control and manipulate the light exposure in their experiments.
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Lab products found in correlation
2 protocols using polygon dmd illuminator
1
Imaging Nurse Cell-Oocyte Ratio
2
Optoelectronic Tweezers for Microscale Manipulation
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The instrument used in this work is similar to one reported previously, [34] featuring a projector interfaced to an upright microscope (Leica DM 2000 with motorized stage
Märzhäuser Scan Plus 100×100). In this work, the projector is a Polygon DMD Illuminator (Mightex Inc.) with two independently controlled LED light sources at 380 nm (UV) and 620 nm (red), respectively, with intensities and other parameters given in the supplementary information. The OET device used in this work comprised a 30 μL fluidic chamber sandwiched in between a top and a bottom plate using 150 µm thick double sided tape (3M 9965). Each plate was formed from a glass slide coated with 200-nmthick ITO, with the bottom plate featuring an additional photoconductive layer of 1 μm thick a-Si:H, deposited by plasma enhanced chemical vapor deposition (PECVD) [28] . In some cases (e.g. Figure 6), metal electrodes were fabricated on top of the a-Si:H layer of the bottom plate (see Supporting
Information and Figure S5 for fabrication details). OET devices were driven by applying a sine-wave bias between the top and bottom plates. For most experiments, the bias was 15 Vpp at 20 kHz; for those involving the manipulation of microcapacitors, the bias varied, but typically was 25 Vpp at 20 kHz.
Märzhäuser Scan Plus 100×100). In this work, the projector is a Polygon DMD Illuminator (Mightex Inc.) with two independently controlled LED light sources at 380 nm (UV) and 620 nm (red), respectively, with intensities and other parameters given in the supplementary information. The OET device used in this work comprised a 30 μL fluidic chamber sandwiched in between a top and a bottom plate using 150 µm thick double sided tape (3M 9965). Each plate was formed from a glass slide coated with 200-nmthick ITO, with the bottom plate featuring an additional photoconductive layer of 1 μm thick a-Si:H, deposited by plasma enhanced chemical vapor deposition (PECVD) [28] . In some cases (e.g. Figure 6), metal electrodes were fabricated on top of the a-Si:H layer of the bottom plate (see Supporting
Information and Figure S5 for fabrication details). OET devices were driven by applying a sine-wave bias between the top and bottom plates. For most experiments, the bias was 15 Vpp at 20 kHz; for those involving the manipulation of microcapacitors, the bias varied, but typically was 25 Vpp at 20 kHz.
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