Anti mouse cd11c n418

Manufactured by Thermo Fisher Scientific

The Anti-mouse CD11c (N418) is a lab equipment product. It is a monoclonal antibody that binds to the CD11c antigen expressed on the surface of mouse dendritic cells.

Automatically generated - may contain errors

Lab products found in correlation

Facsaria, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Cytofix cytoperm buffer, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Anti mouse ifn γ xmg1, by Thermo Fisher Scientific (1 mentions) Anti mouse ctla 4 uc10 4b9, by Thermo Fisher Scientific (1 mentions) Anti mouse cd4 gk1, by BD (1 mentions) Anti mouse foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions) Fixable viability dye efluor 780, by BD (1 mentions) Anti mouse cd8 53 6, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD11c (67 citations) CD11c (N418, (31 citations) Anti-CD11c (N418, (15 citations) Anti-mouse CD11c (8 citations) Hamster anti-mouse CD11c clone N418 PE-Cyanine7 conjugate (2 citations)

2 protocols using anti mouse cd11c n418

Multicolor Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions were prepared from the lung, spleen, bone marrow or blood samples. Cells were incubated with anti-Fc receptor antibodies (clone 2.4G2) and stained with the antibodies on ice for 20 min before washing. For intracellular staining, cells were stained with antibodies to surface molecules, followed by being fixed and permeated in Cytofix/Cytoperm buffer (BD Biosciences). Cells were stained intracellularly and then analyzed using a LSRFortessa (BD Biosciences) or sorted with a FACSAria (BD Biosciences) following the manufacturer’s procedures. Data were analyzed with FlowJo software (TreeStar).
All monoclonal antibodies used for flow cytometry were from eBioscience, unless stated otherwise: anti-mouse CD11b (M1/70), anti-mouse SiglecF (1RNM44N), anti-mouse Ly6C (ER-MP20), anti-mouse Ly6G (1A8-Ly6g), and anti-mouse CD11c (N418).

Zhang J., Liu J., Yuan Y., Huang F., Ma R., Luo B., Xi Z., Pan T., Liu B., Zhang Y., Zhang X., Luo Y., Wang J., Zhao M., Lu G., Deng K, & Zhang H. (2020). Two waves of pro-inflammatory factors are released during the influenza A virus (IAV)-driven pulmonary immunopathogenesis. PLoS Pathogens, 16(2), e1008334.

+ Open protocol

+ Expand

Comprehensive Immunophenotyping of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorochrome-conjugated monoclonal/polyclonal antibodies used in our studies were anti-mouse CD4 (GK1.5, BD Biosciences), anti-mouse CD25 (PC61.5l, eBioscience), anti-mouse Foxp3 (FJK-16s, eBioscience), anti-mouse CD8 (53-6.7, eBioscience), anti-human CD19 (H1B19, eBioscience), anti-human EGFR (AY13, BioLegend), anti-mouse CD45.1 (A20, eBioscience), anti-mouse CTLA-4 (UC10-4B9, eBioscience), anti-mouse neuropilin 1 (eDS304M, eBioscience), anti-mouse Lag-3 (C9B7W, eBioscience), anti-mouse CD11c (N418, eBioscience), anti-mouse IFN-γ (XMG1.2, eBioscience), anti-mouse TNF-α (MP6-XT22, eBioscience), anti-mouse Fas (SA367H8, BioLegend), anti-mouse FasL (MFL3, eBioscience), anti-mouse perforin (eBioMAK-D, eBioscience), anti-mouse GzB (NGZB, eBioscience), anti-mouse GzA (3G8.5, BioLegend), anti-mouse CD107α (1D4B, eBioscience), anti-mouse CD71 (R17217, eBioscience), anti-mouse CPT1a (8F6AE9, Abcam), anti-mouse Glut1 (ER3915, Abcam), and Fixable viability dye eFluor 780 (BD Biosciences). Intracellular staining was performed using the fixation/permeabilization concentrate (catalog 5123-43) and diluent (catalog 5223-56) buffer solutions and IC buffer (catalog 8333-56), according to the eBioscience Foxp3 staining kit. Stained cells were analyzed on an LSR Fortessa flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).

Bolivar-Wagers S., Loschi M.L., Jin S., Thangavelu G., Larson J.H., McDonald-Hyman C.S., Aguilar E.G., Saha A., Koehn B.H., Hefazi M., Osborn M.J., Jensen M.C., Wagner J.E., Pennell C.A, & Blazar B.R. (2022). Murine CAR19 Tregs suppress acute graft-versus-host disease and maintain graft-versus-tumor responses. JCI Insight, 7(17), e160674.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!