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Spectramax 190 spectramax m2e

Manufactured by Molecular Devices
Sourced in United States

The SpectraMax 190 and SpectraMax M2e are multi-mode microplate readers manufactured by Molecular Devices. They are designed to measure the absorbance of samples in microplates, which can be used for various applications such as cell-based assays, enzyme kinetics, and nucleic acid quantification.

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2 protocols using spectramax 190 spectramax m2e

1

Quantification of Physiological Biomarkers

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Physiological biomarkers were assessed in serum using standard in‐house protocol as per the manufacturer's instructions. 17‐β‐estradiol (CAT#B7K720), vitamin B12 (CAT#B7K61E), and 25‐OH vitamin D3 (CAT#B5P020) ELISA‐based kits were obtained from Abbott Diagnostics, USA and estimated using Architect ci 4100, Abbott Diagnostics, USA. CD biomarkers (CD8+CD28−; CAT#564419), TNF‐α (CAT#558273), IL‐1β (CAT#558279), IL‐8 (CAT#558277), and IL‐6 (CAT#558276) ELISA‐based kits were obtained from BD Biosciences, USA and estimated by FACS Calibur, BD Biosciences. Vitamin C (CAT#E‐EL‐0011), 1, 25 dihydroxy vitamin D3 (CAT#E‐EL‐0016), oxytocin (CAT#E‐EL‐0029), insulin, klotho (CAT#E‐EL‐H5451), oxidized LDL (CAT#E‐EL‐H0124), isoprostane (CAT#E‐EL‐0041), norepinephrine (CAT#E‐EL‐0047), dopamine (CAT#E‐EL‐0046), and acetylcholine (CAT#E‐EL‐0081) ELISA‐based kits were obtained from Elabscience Biotechnology Inc., and estimated by SpectraMax 190/SpectraMax M2e, Molecular Devices.8
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2

Comprehensive Biomarker Profiling Protocol

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Physiological biomarkers were assessed in serum using in-house protocol as per manufacturer's instructions. 17-β-estradiol (CAT#B7K720) and 25-OH vitamin D3 (CAT#B5P020) ELISA-based kits were obtained from Abbott Diagnostics, USA and measured using Architect ci 4100, Abbott Diagnostics, USA. TNF-α (CAT#558273), IL-1β (CAT#558279), IL-8 (CAT#558277) ELISA-based kits were obtained from BD Biosciences, USA and measured by FACS Calibur, BD Biosciences, USA. Vitamin C (CAT#E-EL-0011), 1, 25 dihydroxy vitamin D (CAT#E-EL-0016), oxytocin (CAT#E-EL-0029), klotho (CAT#E-EL-H5451), oxidized LDL (CAT#E-EL-H0124), malondialdehyde (CAT#E-EL-0060), norepinephrine (CAT#E-EL-0047), dopamine (CAT#E-EL-0046), and acetylcholine (CAT#E-EL-0081) ELISA-based kits were obtained from Elabscience Biotechnology Inc. USA and determined by SpectraMax 190/SpectraMax M2e, Molecular Devices, USA.
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