Coated monofilament

Manufactured by Doccol

Sourced in United States

Coated monofilament is a type of lab equipment used for various applications. It consists of a single filament or strand that has been coated with a specific material. The coating can serve different purposes, such as enhancing durability, chemical resistance, or surface properties. This product is designed to meet specific requirements in laboratory settings.

Automatically generated - may contain errors

Lab products found in correlation

Protein marker, by Thermo Fisher Scientific (3 mentions) Isoflurane, by Merck Group (3 mentions) Uvp biospectrum imaging system, by Analytik Jena (3 mentions) Polyvinylidene difluoride pvdf membrane, by Merck Group (3 mentions) Ez ecl kit, by Sartorius (3 mentions) Tween 20, by Merck Group (3 mentions) Chloral hydrate, by Sinopharm (3 mentions) β actin, by MultiSciences Biotech (3 mentions)

2 protocols using coated monofilament

Permanent Middle Cerebral Artery Occlusion

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Fifty eight animals were weighed and anesthetized with an intraperitoneal administration of Ketamine (100 mg/kg) and Xylazine (10 mg/kg). This was followed by a subcutaneous injection of Piritramide (15 mg/kg). Animals were then intubated with an 18G intravenous catheter (BD Biosciences) and anesthesia was maintained throughout surgery with 1.5% isoflurane delivered in 1.5 L air and 0.8 L oxygen per minute. Body temperature was regulated using a heating pad (Trixie 76085 Heizmatte, Trixie, Flensburg, Germany). Permanent middle cerebral artery occlusion (MCAO) of the right hemisphere was performed according to the suture model described by Zea Longa
et al. using a coated monofilament (Doccol Corporation, CA, USA) (
Longa
et al., 1989). Briefly, a 3 cm coated monofilament with a thickened tip was inserted into the external carotid artery (ECA) and advanced to the middle cerebral artery (MCA) to induce ischemia in the MCA territory.

Altmann P., Mildner M., Haider T., Traxler D., Beer L., Ristl R., Golabi B., Gabriel C., Leutmezer F, & Ankersmit H.J. (2014). Secretomes of apoptotic mononuclear cells ameliorate neurological damage in rats with focal ischemia. F1000Research, 3, 131.

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Protein Detection and Analysis Protocol

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Isoflurane, Tween 20, and 2,3,5-trihenylterzolium chloride (TTC) were purchased from Sigma-Aldrich (St. Louis, USA). Coated monofilament was purchased from Doccol Corporation (Sharon, USA). Chloral hydrate was purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). Protein marker was purchased from Fermentas (Waltham, USA). Polyvinylidene difluoride (PVDF) membrane was purchased from Merck Millipore (Burlington, USA). Primary antibodies of SPK1 (sc-22702) and SPK2 (sc-22704) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). β-actin and IgG secondary antibody were purchased from Multi Sciences (Hangzhou, China). EZ-ECL kit was purchased from Biological Industries (Beit HaEmek, Israel); PH Indicator was purchased from Leici Device Works (Shanghai, China). A 5417R High-speed Freezing Centrifuge was purchased from Eppendorf (Hamburg, Germany). A 752 Ultraviolet Spectrophotometer was purchased from Puxi General Instruments (Beijing, China). A DYY-4C electrophoresis system was purchased from Tanon Science & Technology (Shanghai, China). A UVP BioSpectrum Imaging System was purchased from UVP (Upland, USA).

Ge J., Kong K., Cheng X., Wu T., Liu D., Peng L., Shen Z., Lu G, & Shen Y. (2020). Isoflurane cerebral preconditioning in a spontaneous hypertension rat model is associated with sphingosine kinases. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 29(6).

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