Methylglyoxal mgo solution

Water filtered using 0.20 μm pore sized filters was used in all analyses. HPLC-grade acetonitrile (ACN) was obtained from RCI Labscan, Bangkok, Thailand. Hydroxyacetone (HA) (90%) and methylglyoxal (MGO) solution (40% w/w in water) were obtained from Sigma-Aldrich, Castle Hill, New South Wales, Australia. o-(2,3,4,5,6-Pentafluorobenzyl) hydroxylamine hydrochloride (PFBHA) (99%) was obtained from Alfa Aesar, Gymea, NSW, Australia. Spectra/Por^® Dialysis Membrane (MWCO: 3500) was sourced from Repligen, Waltham, MA, USA. NaCl and KCl were purchased from Chem Supply Pty Ltd., South Australia, and Na₂HPO₄ and KH₂PO₄ were obtained from Ajax Finechem, New South Wales, Australia.

Chemicals and reagents, and their sources: 4,5,7-trihydroxyflavanone (Alfa Aesar, Lancaster, UK), anhydrous sodium sulphate (Merck KGaA, Darmstadt, Germany), low viscosity sodium alginate (BÜCHI Labortechnik AG, Meierseggstrasse 40, 9230 Flawil, Switzerland), and anhydrous magnesium sulphate (Scharlab S.L., Sentmenat, Barcelona, Spain). Hydroxyacetone (HA) (90%) and methylglyoxal (MGO) solution (40% w/w in water) were purchased from Sigma-Aldrich, Castle Hill, NSW, Australia. O-(2,3,4,5,6-Pentafluorobenzyl) hydroxylamine hydrochloride (PFBHA) (99%) was sourced from Alfa Aesar, Gymea, NSW, Australia.
Solvents and their sources: Methanol (Scharlau, Barcelona, Spain), dichloromethane (Merck KGaA, Darmstadt, Germany), acetonitrile (RCI Labscan, Bangkok, Thailand), toluene (APS Chemicals, Sydney, NSW, Australia), and ethyl acetate and formic acid (85%) (Ajax Finechem Pvt Ltd., Sydney, NSW, Australia). Sterile deionised water was used throughout to prepare the formulations.