EV preparations were resuspended in 20 μL PBS (pH 7.4) and fixed by adding an equal volume of 2% paraformaldehyde in 0.1 mol/L phosphate buffer (pH 7.4). EVs were then adsorbed for 20 min to Formvar–carbon-coated copper grids by floating the grids on 5 μL drops on parafilm. Subsequently, grids were rinsed in PBS and negatively stained with 2% uranyl acetate for 5 min at RT [44 (link)]. Stained grids were embedded in 2.5% methylcellulose for improved preservation and air dried before examination. Electron micrographs were taken at Hitachi TEM microscope (HT7800 series, Tokyo, Japan) equipped with Megaview G3 digital camera and Radius software (EMSIS, Muenster, Germany).
Tem microscope ht7800 series
Manufactured by EMSIS
Sourced in Japan
The TEM microscope (HT7800 series) is a transmission electron microscope designed for high-resolution imaging and analysis of samples at the nanoscale. It features advanced optics and electronics to provide clear and detailed images of the internal structure and composition of materials.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using tem microscope ht7800 series
1
Electron Microscopy of Extracellular Vesicles
2
Electron Microscopy of Extracellular Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Electron microscopic analysis was performed on isolated vesicle preparations as follows. The pellets were resuspended in 20 μL filtered D-PBS (pH 7.4) and fixed by adding an equal volume of 2% paraformaldehyde in 0.1 mol/L phosphate buffer (pH 7.4). EV preparations were first adsorbed for 10 min to formvar-carbon coated copper grids by floating the grids on 5 μl drops on parafilm. Grids with adhered vesicles were then rinsed in D-PBS and negatively stained with 2% uranyl acetate for 5 min at room temperature. Subsequently, grids were embedded in 2.5% methylcellulose for improved preservation and air dried before examination. Electron micrographs were taken at Hitachi TEM microscope (HT7800 series, Tokyo, Japan) equipped with Megaview 3 digital camera and Radius software (EMSIS, Germany).
3
Negative Staining of Extracellular Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EVs were resuspended in 20 μl dPBS and fixed by adding an equal volume of 2% paraformaldehyde in 0.1 mol/l phosphate buffer (pH 7.4), as previously described [47] . EVs were then adsorbed for 10 min to formvar-carbon coated copper grids by floating the grids on 5 μl drops on parafilm. Subsequently, grids with adhered vesicles were rinsed in PBS and negatively stained with 2% uranyl acetate for 5 min at room temperature. Stained grids were embedded in 2.5% methylcellulose for improved preservation and air dried before examination. Electron micrographs were taken at Hitachi TEM microscope (HT7800 series, Tokyo, Japan) equipped with Megaview 3 digital camera and Radius software (EMSIS, Germany). To visualize EV size distribution, the results were plotted as colorblind safe scatter dot plot in which each size measured is represented as a point along with lines for the median value and the range.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!