Hplc grade syringe filters

Chlorinated ethenes and ethene were measured by injecting 100 μL headspace gas from the cell cultures on an Agilent 7890A gas chromatograph equipped with a flame ionization detector and a 30-m J&W capillary column with an inside diameter of 0.32-mm (Agilent Technologies, USA). A gradient temperature program method was used as previously described.^{40 (link)}Measurement of soluble As(III) and As(V) was performed using an Agilent 1260 Infinity high-performance liquid chromatograph (HPLC) (Agilent Technologies, USA) equipped with an Aminex HPX-87H Ion Exclusion Column (300mm x 7.8mm, BIO-RAD, USA) coupled to a Hamilton PRP-X300 Reversed-Phase Column (250mm x 4.1mm, Hamilton Co., USA) and an Agilent 7700 series inductively coupled plasma mass spectrometer (ICP-MS) (Agilent Technologies, USA) as previously described.⁴¹ Arsenous acid, arsenic acid, monomethylarsonate, dimethylarsinate, and arsenobetaine are quantifiable using the applied method.⁴¹ Samples consisting of 0.5 mL liquid medium were collected at three timepoints during the experiment (day 0, day 12, and day 17) from both As(III)- and As(V)-amended cultures. Liquid medium samples were filtered using 0.2 μM HPLC-grade syringe filters (Pall Life Sciences, England) and diluted to obtain analytical concentrations within standard solution calibration.