The pH and qualitative protein results contained in the urinalysis strips were reported by the fully automated UC-3500 (Sysmex, Kobe, Japan). Data are presented in the reports on an ordinal scale (as “normal,” “negative,” “positive,” or “nominal concentrations”).
Uc 3500
Manufactured by Sysmex
Sourced in Japan
The UC-3500 is a fully automated urine particle analyzer developed by Sysmex. It performs quantitative analysis of various urine particles, including red blood cells, white blood cells, epithelial cells, and casts. The UC-3500 utilizes advanced optical and electrical impedance technologies to provide accurate and reliable results for clinical laboratory testing.
Automatically generated - may contain errors
Lab products found in correlation
10 protocols using uc 3500
1
Automated Urinalysis Analysis
2
Comprehensive Urinalysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The urine samples were collected early in the morning and were processed using an automated urine chemistry analyzer (UC3500, Sysmex, Kobe, Japan) and a fluorescence flow cytometer (UF 1000i, Sysmex, Kobe, Japan) according to the instructions of the manufacturer. If necessary, we carried out an optical microscope examination. Instructions were recommended for each subject before urine collection: (1) wash hands thoroughly with soap and water and dry them with a cloth; (2) thoroughly wash the genital organs with soap and water and dry them and (3) eliminate the first flow of urine of the W.C. and collect the second one in the sterile container without interrupting urination. The following parameters were analyzed on urine samples: pH (5.5–7 mg/dL), a specific weight (1005–1030), colour, appearance, presence of bacterial cells (0–1000 n/µL), presence of squamous cells (0–20 n/µL), leukocytes (0–18 n/µL), erythrocytes (0–14 n/µL), proteins (0 e 20 mg/dL), glucose, ketones, bilirubin, haemoglobin, nitrite and leukocyte esterase.
3
Ketonuria in SGLT2 Inhibitor Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patients were classified into two groups: those with and without ketonuria. The group with ketonuria included subjects who had no previous history of ketonuria prior to starting SGLT2 inhibitor treatment and those who developed ketonuria coinciding with the treatment period. Only these patients were selected for inclusion in the study. Patients who had ketonuria before beginning SGLT2 inhibitor therapy were excluded. Additionally, those who developed ketonuria during the treatment period but did not have persistent ketonuria throughout were also excluded (Supplemental Fig. S1). Ketone bodies were detected using the sodium nitroprusside reaction (UC-3500, Sysmex Corporation, Kobe, Japan).
Clinical and biochemical data were collected for analysis from the initial period and at 6 months following the initiation of SGLT2 inhibitor treatment. The degree of improvement in eGFR after 6 months and 1 year of SGLT2 inhibitor treatment was evaluated and compared with baseline values.
Clinical and biochemical data were collected for analysis from the initial period and at 6 months following the initiation of SGLT2 inhibitor treatment. The degree of improvement in eGFR after 6 months and 1 year of SGLT2 inhibitor treatment was evaluated and compared with baseline values.
4
Comprehensive Safety Assessments in Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Safety laboratory assessments were performed at the Department of Medical Biochemistry (Oslo University Hospital Rikshospitalet, Oslo, Norway), and included platelet count, red blood cell (RBC) count and indices, haemoglobin, haematocrit, white blood cell (WBC) count, iron variables (iron, ferritin, transferrin), blood urea nitrogen, creatinine, fasting glucose, insulin, C-peptide, potassium, sodium, calcium, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyltransferase (GGT), lactate dehydrogenase (LD), creatine kinase (CK), bilirubin, C-reactive protein, total protein and lipids, as assessed by colorimetric and/or enzymatic methods (Cobas c702 analyser, Roche Diagnostics International Ltd, Rotkreuz, Switzerland). Urine pH, glucose, protein, blood, ketones, bilirubin, urobilinogen, nitrite and leukocyte esterase were measured by an automated urine test strip reader (UC-3500 Sysmex, Kobe, Japan); urine RBC and WBC counts were assessed by a urine particle analyser (UF-5000 Sysmex, Kobe, Japan).
5
Urine Analysis of Athlete Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
First-morning urine samples were collected from athletes and analyzed within 4 h of arrival by the combined use of an automated urine chemistry analyzer (UC3500, Sysmex, Kobe, Japan) and a fluorescence flow cytometer (UF 1000i, Sysmex, Kobe, Japan) according to the manufacturer’s instructions. Where necessary, we conducted an examination using an optical microscope.
6
Automated Urine Analysis: Sysmex UN Series
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Sysmex UN series fully automated urine analyser is a new-generation urine analyser developed by Sysmex Corporation (Kobe, Japan). It is a modular system that integrates three main modules: UC-3500 (physical and chemical analyser), UF-4000 (particle analyser), and UD 10 (digital particle screening device). Each module can be used as a standalone urine analyser or integrated as a complete automated urine work area. The Sysmex UC-3500 is a urine test strip analyser that employs reflectance photometry, refractometry, and spectrophotometry to analyse urine chemical and physical properties. The UF-4000 operates based on the fluorescent flow cytometry principle to identify, classify, and quantify urine particles. The classification and quantification of urine particles are based on the sizes, shapes, and staining features of the particles [16 ].
7
Urinary Microalbumin Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urine samples from individual mice were collected at 20 weeks as described previously, and the concentration of urinary microalbumin was analysed via an automatic chemistry analyser (SYSMEX UC3500).
8
Comprehensive Metabolic Profile Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three-milliliter blood samples were collected from each of the subjects into BD Vacutainer® K2EDTA tubes (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) to analyze HbA1c levels on a Tosoh G8 HPLC Analyzer, ion-exchange HPLC instrument [NGSP-certified, anchored to the Diabetes Control and Complication Trial (DCCT) reference study and to the IFCC Reference Method].
Glucose concentration was measured from serum collected in BD Vacutainer® serum separating tubes II Advance Tube (SST) (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) using the hexokinase method with a Cobas 8000® Chemistry System (Roche Diagnostics, Basel, Switzerland).
Cholesterol, cHDL and triglyceride levels were also measured (Cobas 8000, Roche, Mannheim, Germany) in serum samples through an enzymatic colorimetric method, and cLDL was calculated using the Friedewald formula.
The quantitative urinary albumin was measured (Cobas 8000, Roche, Mannheim, Germany) with an immunoturbidimetric assay. The albumin-to-creatinine ratio (ACR) strip test is based on the protein error of the pH indicator (tetrabromophenol blue) and measured in UC-3500 (Sysmex, Kobe, Japan).
Glucose concentration was measured from serum collected in BD Vacutainer® serum separating tubes II Advance Tube (SST) (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) using the hexokinase method with a Cobas 8000® Chemistry System (Roche Diagnostics, Basel, Switzerland).
Cholesterol, cHDL and triglyceride levels were also measured (Cobas 8000, Roche, Mannheim, Germany) in serum samples through an enzymatic colorimetric method, and cLDL was calculated using the Friedewald formula.
The quantitative urinary albumin was measured (Cobas 8000, Roche, Mannheim, Germany) with an immunoturbidimetric assay. The albumin-to-creatinine ratio (ACR) strip test is based on the protein error of the pH indicator (tetrabromophenol blue) and measured in UC-3500 (Sysmex, Kobe, Japan).
9
Quantitative Urinary Albumin-Creatinine Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Quantitative analysis of albumin and creatinine was performed on a Cobas C 501 analyzer (Roche Diagnostics GmBH, Mannheim, Germany). Albumin in urine samples was measured using the bromocresol green colorimetric method. Urine creatinine levels were measured by an enzymatic colorimetric method that was ID/MS traceable. The ACR was calculated. A Sysmex UC-3500 automatic urine analyzer was used to evaluate ACR with a semi-quantitative method. For albumin, the dipstick analysis (Meditape UC-11A, Sysmex Corporation HQ: Kobe, Japan) is based on a PH indicator (tetra bromophenol blue); for creatinine, the method is based on the Benedict-Behre method.
10
Urine Analysis for Acute Kidney Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The urine samples were collected in containers, transported and analysed within 2 h of collection. Samples were analysed by automated urine analyser UC-3500(Sysmex, EuropeGmbH) for chemical analysis and UF-5000(Sysmex, EuropeGmbH) for analysing the urine sediment. A further microscopic analysis of sediments was performed, if required. Urine sediments were prepared by centrifuging 5 ml of urine for 5 min at 1500 rotations per minute. After discarding the supernatant, a drop of the sediment was placed on the slide covered using cover slip and examined under microscope. Presence of 0–3 RBC’s/high power field and 0–5 pus cells/high power field were considered normal and counts above these levels were reported as positive for microscopic haematuria and pyuria respectively. Trace proteinuria was considered as negative. The baseline and peak serum creatinine was recorded. The AKI was diagnosed based on the KDIGO criteria [12 ]. The outcome studied was hospital mortality. The study was approved by the institutional Ethical Committee.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!