Enriched PBMCs were resuspended in RPMI 1640 (Solarbio; Beijing, China), supplemented with 10% FBS. Cells were stimulated with recombinant human IL-7 (50 ng/mL) (Beyotime; Shanghai, China) in the presence or absence of 150 nM tofacitinib (Meilun Biotechnology; Dalian, China) and cultured for up to 4 days in 96-well plates.
Tofacitinib
Manufactured by Meilun
Sourced in China
Tofacitinib is a lab equipment product that serves as a Janus kinase (JAK) inhibitor. It functions by blocking the activity of one or more of the JAK enzymes, which play a role in immune system regulation.
Automatically generated - may contain errors
2 protocols using tofacitinib
1
IL-7 Stimulation of Enriched PBMCs
2
Tofacitinib Modulates Immune Responses
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Tofacitinib was purchased from Dalian Meilun Biotechnology Co., Ltd. ConA and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich; Merck KGaA. The antibodies used for western blotting and immunohistochemistry in this study were purchased from Santa Cruz Biotechnology, Inc., R&D Systems, and Cell Signaling Technology, Inc., including antibodies against STAT1, phosphorylated STAT1 (p-STAT1), TNF-α, and IFN-γ. The antibodies used for flow cytometry, such as those recognizing CD4, CD25, Foxp3, and IL-17A were purchased from BioLegend, Inc. and BD Pharmingen; BD Biosciences. Fetal bovine serum (FBS) was purchased from Gibco; Thermo Fisher Scientific, Inc.
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