Acyclovir

Manufactured by Meilun

Sourced in China

Acyclovir is a laboratory reagent used for research purposes. It is a synthetic nucleoside analogue that exhibits antiviral activity against certain viruses, including herpes simplex viruses. Acyclovir functions by interfering with viral DNA replication, thereby inhibiting viral proliferation.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Chloroquine, by Meilun (2 mentions) Vero cell, by American Type Culture Collection (1 mentions) Hsv 2 strain g, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions) Pepsin, by Avantor (1 mentions) Trypsin, by Avantor (1 mentions) Api 4000, by AB Sciex (1 mentions) 1200 series, by Agilent Technologies (1 mentions) Avance 400, by Bruker (1 mentions) Potassium dihydrogen phosphate, by Sinopharm (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Solarbio (1 mentions) Disodium hydrogen phosphate, by Sinopharm (1 mentions) Sodium dihydrogen phosphate, by Sinopharm (1 mentions) Hplc grade ammonium acetate, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions)

5 protocols using acyclovir

Antiviral Activity Assessment of Retro-2.1

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African green monkey kidney cells (Vero cells) were obtained from ATCC (Cat. No. CCL-81) and grown in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, USA) (DMEM-10% FBS) at 37°C with 5% CO 2 .
HSV-2 strain G was obtained from ATCC (Cat. No. VR-734) (KU310668). Its propagation and titer determination were performed in Vero cells.
Retro-2.1 (6-fluoro-1-methyl-2-(5-(2-methylthiazol-4-yl)thiophen-2-yl)-3-phenyl-2,3-dihydroquinazolin-4(1H)-one) (Fig. 1) with a purity higher than 95% determined by LC/MS was synthesized in-house [24] . Chloroquine and acyclovir, which were used as positive control drugs as reported, with purities higher than 98% as determined by HPLC, were purchased from Meilun Biotech Co., Ltd. (China) [28, 29] . The compounds were dissolved in dimethyl sulfoxide as stocks and diluted with DMEM supplemented with 2% FBS (DMEM-2% FBS) to working concentrations.

Dai W., Wu Y., Bi J., Wang J., Wang S., Kong W., Barbier J., Cintrat J.C., Gao F., Jiang Z., Gillet D., Su W, & Jiang C. (2018). Antiviral Effect of Retro-2.1 against Herpes Simplex Virus Type 2 In Vitro. Journal of microbiology and biotechnology, 28(6).

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Cytarabine Nanoparticle Development and Evaluation

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Cytarabine (Ara-C) was purchased from the Aladdin Industrial Corporation. Palmitic acid (PA), sodium hydroxide, potassium dihydrogen phosphate, sodium dihydrogen phosphate and disodium hydrogen phosphate were bought from Sinopharm Chemical Reagent Co., Ltd. Ethyl chloroformate (EtOCOCl) was obtained from Chengdu Beisite Reagent Co., Ltd. Pepsin (1 : 3000) and trypsin (1 : 250) were purchased from Amresco and Sangon Biotech (Shanghai) Co., Ltd, respectively. Acyclovir (HPLC > 98%) was purchased from Dalian Meilun Biotech Co., Ltd. HL60 and K562 cells were kindly provided by the Immunopharmacology Institute of Shandong University and the Shandong Analysis and Test Center. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Beijing Solarbio Technology Co., Ltd.
The spectroscopy equipment was: an NMR spectrometer (¹H-NMR, Bruker Avance 400), an electrospray tandem mass spectrometer (MS, AB SCIEX API 4000), an FTIR spectrometer (Nicolet 6700), a transmission electron microscope (TEM, JEM-200CX), a high performance liquid chromatograph (HPLC, Agilent Technologies 1200 Series), an HPLC-MS (Agilent 1260 triple quadrupole mass spectrometer equipped with an ESI source), and a microplate reader (ELISA, PerkinElmer).

Zhang J., Zhang D., Hu X., Liu R., Li Z, & Luan Y. (2018). Rational design of a new cytarabine-based prodrug for highly efficient oral delivery of cytarabine. RSC Advances, 8(24), 13103-13111.

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FHND6081 Bioactivation and Quantification

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FHND6091 was prepared by Jiangsu Chia Tai Fenghai Pharm. Co. LTD and hydrolyzed immediately to the biologically active form FHND6081 on exposure to aqueous solutions or plasma. The internal standard 4-Hydroxy mephenytoin (MP) was purchased from Toronto Research Chemicals, Inc (Toronto, Canada). Acyclovir (purity, 99%), Minoxidil (purity, 99%) and GF120918 (purity, 99%) were purchased from Dalian Meilun biotechnology Co., Ltd, whereas digoxin (purity, 98%) was purchased from MCE Technologies, USA. All compounds were stored at −20°C. HPLC grade ammonium acetate and acetonitrile were purchased from Merck, Germany.

Yang X., Liu A., Yang L., Wen T., Wang J., Shi J., Zhou H., Chen Z., Lei M, & Zhu Y. (2022). Preclinical Pharmacokinetics, Tissue Distribution and in vitro Metabolism of FHND6091, a Novel Oral Proteasome Inhibitor. Drug Design, Development and Therapy, 16, 3087-3107.

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Leflunomide, Teriflunomide, and Probenecid Assay

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Leflunomide (LEF, > 98.5%), teriflunomide (TER, > 99%), probenecid, acyclovir and G418 were obtained from Meilun Biology Technology (Dalian, China), and 6-carboxyfluorescein (6-CF) was obtained from Sigma–Aldrich (St. Louis, MO, USA). MK571, the sodium salt (98%), was purchased from CSNpharm (Shanghai, China). TRIzol reagent, 0.05% trypsin-EDTA, DMEM high glucose, and Hanks’ balanced salt solution (HBSS) were purchased from Invitrogen (Carlsbad, CA, USA). The BCA protein assay kit was from Pierce Chemical (Rockford, IL, USA). All other reagents and solvents were of analytical grade and commercially available.

Liao X.Y., Deng Q.Q., Han L., Wu Z.T., Peng Z.L., Xie Y., Wang G.J., Aa J.Y, & Pan G.Y. (2019). Leflunomide increased the renal exposure of acyclovir by inhibiting OAT1/3 and MRP2. Acta Pharmacologica Sinica, 41(1), 129-137.

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HSV-2 Inhibition Assay in Vero Cells

Cited 3 times

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African green monkey kidney cells (Vero cells) obtained from American type culture collection (ATCC) (cat # CCL-81) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) (DMEM-10% FBS) at 37 °C with 5% CO₂.
HSV-2 strain G obtained from ATCC (cat # VR-734) (KU310668) was propagated in Vero cells. Virus titration was performed by endpoint dilution and plaque assays.
ABMA [1-adamantyl (5-bromo-2-methoxybenzyl) amine] (Figure 1) was synthesized in-house. The positive control drugs, chloroquine and acyclovir, were purchased from Meilun Biotech Co., Ltd. (Dalian, China) [19 (link),20 (link),21 (link),22 (link),23 (link),24 (link)]. The purities of the compounds were higher than 98%, as determined by High Performance Liquid Chromatography (HPLC). The compounds were dissolved in dimethyl sulfoxide (DMSO) as stock solutions.
Specific-pathogen-free female BALB/c mice (6–8 weeks old) were obtained from the Changchun Institute of Biological Products and maintained under the guidelines for animal experiments at Jilin University, China.

Dai W., Wu Y., Bi J., Wang S., Li F., Kong W., Barbier J., Cintrat J.C., Gao F., Gillet D., Su W, & Jiang C. (2018). Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo. Viruses, 10(3), 119.

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