Strept avidin biotin complex immunohistochemical kit

NBP was provided by Shiyao Group Pharmaceutical Co., Ltd. (Shijiazhuang, China). BDNF, TrkB, p-AKT and caspase-3, terminal deoxynucleotidyl transferase-mediated nick end-labelling (TUNEL) kit, Strept-Avidin-Biotin Complex (SABC) immunohistochemical kit and Western blot reagents were supplied by Boster Biological Technology Co., Ltd. (Wuhan, China). DAB (3,3'-diaminobenzidine) chromogenic reagent kit, wood grain and eosin were supplied by the Department of Pathology, the Seventh Affiliated Hospital. BCA protein quantitative kit, cracking liquid RIPA organization and enhancement fixing kits were purchased from Blue Skies Biotechnology Research Institute (Shanghai, China). Rabbit anti-β-tubulin antibody, goat anti-rabbit IgG (H + L)-HRP conjugate, 20× LumiGLO Reagent and 20× peroxide were purchased from Cell Signaling Corporation (Shanghai, China). Predye protein Marker (Fermentas, Lithuania), Trizol and real-time polymerase chain reaction (qPCR) kit were purchased from Promega Corporation (Shanghai, China). BDNF, TrkB, β-actin primary antibody and related secondary antibodies were purchased from Sigma Chemical Company (St. Louis, MO, USA). BDNF, TrkB, AKT, caspase-3 and β-actin primers were synthesized by Shanghai Shengon Biological Engineering Co., Ltd. (Shanghai, China).