Migration assays were performed using 8.0 μM polycarbonate transwell inserts (Corning) according to manufacturer's instructions. Briefly, the bottom wells were filled with 600 μL medium containing 10% bovine serum. A total of 1×105 cells in 100 μl serum-free medium were plated in the upper inserts and allowed to migrate for 24 hrs. Non-migrated cells were removed with a cotton swab, while cells that had migrated were fixed and stained with Hema-3 (Fisher Scientific) for counting. The fold change of cell migration was calculated by comparing the cells expressing miR-9 and HPV E6 with the cells expressing negative control RNA and empty vehicle vector, respectively.
8.0 μm polycarbonate transwell inserts
Manufactured by Corning
The 8.0 μM polycarbonate transwell inserts are a laboratory equipment product designed for cell culture applications. The core function of these inserts is to provide a permeable membrane that allows for the exchange of media, nutrients, and signaling molecules between the upper and lower chambers of a transwell system.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 8.0 μm polycarbonate transwell inserts
1
Cell Migration Assay Protocol
2
Cell Migration Assay Using Transwell
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