ATP and ADP concentrations were measured by means of ATP Bioluminescence Assay Kit HS II (Roche Applied Science, Monza, Italy) and the ADP Colorimetric/Fluorometric Assay Kit (Sigma-Aldrich). Cells were harvested, counted, and lysed with the above-mentioned lysis buffer. Aliquots (50 µL) from each diluted sample or standard were transferred to 96-well plates, after which 50 µL of a luciferase reagent was added. After mixing, the emitted light was measured and integrated over 10 s on an automated microplate luminometer (Bio-Tek, San Diego, CA, USA).
Automated microplate luminometer
Manufactured by Agilent Technologies
Sourced in United States
The Automated Microplate Luminometer is a laboratory instrument designed to measure luminescence from microplate samples. It automates the process of detecting and quantifying light emission, providing precise and consistent results.
Automatically generated - may contain errors
2 protocols using automated microplate luminometer
1
ATP and ADP Quantification Assay
2
Quantifying Intracellular Heme Levels
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Intracellular heme levels were measured using a heme colorimetric assay kit (BioVision, Mountain View, CA, United States). Briefly, animal tissues were lysed in lysis buffer and homogenized using sonication. Aliquots (50 μL) from each diluted sample or standard were transferred into 96-well plates prior to the addition of 50 μL of heme assay kit solution to each well. After incubation for 30 min, the absorbance was measured at 570 nm using an automated microplate luminometer (BioTek, San Diego, CA, United States).
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