The samples are first fixed in paraformaldehyde, followed by incubation in milk to prevent non-specific binding. To stain the actin and cytoskeleton, the samples are incubated with Alexa Fluor 488(Life Technologies) followed by DAPI (4′, 6′-diamidino-2-phenylindole, Life Technologies) to stain the nucleus. An epi-fluorescent Nikon E-400 microscope was used and the data were recorded by a DS-5M-U1 color digital camera (Nikon, Canada).
Ds 5m u1 color digital camera
Manufactured by Nikon
Sourced in Canada
The Nikon DS-5M-U1 is a color digital camera. It is designed for use in laboratory environments.
Automatically generated - may contain errors
3 protocols using ds 5m u1 color digital camera
1
Fluorescent Staining of Cytoskeleton
2
Cytoskeleton and Nucleus Staining Protocol
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In the first step, paraformaldehyde was used to fix the samples. Then it was followed by incubation in skimmed milk powder to prevent non-specific binding. The samples are then incubated with Alexa Fluor 488 phalloidin (Life Technologies) to stain the actin and cytoskeleton followed by DAPI (4′, 6′-diamidino-2-phenylindole, Life Technologies) to stain the nucleus. An epi-fluorescent Nikon E-400 microscope with FITC and DAPI filter was used, and the data were recorded using a DS-5M-U1 color digital camera (Nikon, Canada).
3
Fluorescent Cytoskeleton and Nuclear Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In the first step, paraformaldehyde was used to fix the samples. Then it was followed by incubation in skimmed milk powder to prevent non-specific binding. The samples are then incubated with Alexa Fluor 488 phalloidin (Life Technologies) to stain the actin and cytoskeleton followed by DAPI (4′, 6′-diamidino-2-phenylindole, Life Technologies) to stain the nucleus. An epifluorescent Nikon E-400 microscope with FITC and DAPI filter was used, and the data were recorded using a DS-5M-U1 color digital camera (Nikon, Canada).
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