Uhplc agilent 1290 infinity 2 6470

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1290 Infinity II 6470 is a high-performance ultra-high performance liquid chromatography (UHPLC) system. It is designed for fast and efficient separation of complex samples, providing high-resolution chromatography. The system features advanced technology and components to deliver accurate and reliable results.

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Lab products found in correlation

Masshunter workstation, by Agilent Technologies (4 mentions) Protein assay, by Bio-Rad (1 mentions)

Close spelling variants of this product

Agilent 1290 Infinity II UHPLC 1290 Infinity II-6470 Agilent 1290 Infinity UHPLC 1290 Infinity II UHPLC Agilent 1290 Infinity II Agilent 1290-6470 1290 Infinity UHPLC Agilent 1290 UHPLC Agilent 1290 Infinity 1290 Infinity II

4 protocols using uhplc agilent 1290 infinity 2 6470

Quantification of Quinidine and Hydroxyquinidine in Plasma

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QND and H-QND plasma levels were measured at the Laboratory of Metabolic Diseases and Drug Biology at Bambino Gesù Children’s Hospital in Rome. Liquid chromatography and mass spectrometry analyses were performed by using a UHPLC Agilent 1290 Infinity II 6470 (Agilent Technologies, Santa Clara, CA, USA) equipped with an ESI-JET-STREAM source operating in the positive ion (ESI+) mode. The software used for controlling this equipment and analyzing data was MassHunter Workstation (Agilent Technologies). The assay calibration curves were linear and ranged from 0.025 to 7.60 µg/mL for QND and from 0.025 to 12.60 for H-QND. Each batch of patients’ analyses included both low- and high-quality controls (QCs) at fixed concentrations. For QND, L-QC and H-QC were 0.82 and 5.12 µg/mL, respectively. For H-QND, L-QC and H-QC were 1.15 and 8.65 µg/mL, respectively. Calibrators, QCs, and samples were analyzed using a validated LC-MS/MS kit (MassTox^® TDM Anti-arrhythmic drugs) provided by Chromsystems (Chromsystems Instruments & Chemicals GmbH, Gräfelfing, Germany).
This kit included calibrators and QCs, and was validated according to EMA guidelines for bioanalytical methods validation:
(http://www.ema.europa.eu/docs/en_GB/document_library/Scientific_guideline/2011/08/WC500109686.pdf, 21 July 2011).

Ferretti A., Simeoli R., Cairoli S., Pietrafusa N., Trivisano M., Dionisi Vici C., Specchio N, & Goffredo B.M. (2022). Therapeutic Drug Monitoring of Quinidine in Pediatric Patients with KCNT1 Genetic Variants. Pharmaceutics, 14(10), 2230.

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Dexmedetomidine Quantification Protocol

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The coordinating center for the current Italian TREX is IRCCS Istituto Giannina Gaslini. Eight centres are participating this TREX in Italy. Plasma was separated by centrifugation (2500 g for 10 min at 4°C) and stored at −80°C at the site until assay. The assay was performed by an accredited central laboratory, located at IRCCS Ospedale Pediatrico Bambino Gesù in Rome (Italy). Liquid chromatography and mass spectrometry analysis were performed by a UHPLC Agilent 1290 Infinity II 6470 (Agilent Technologies) equipped with an ESI‐JET‐STREAM source operating in the positive ion (ESI+) mode for Dexmedetomidine. The software used for controlling this equipment and analyzing data was MassHunter Workstation (Agilent Technologies).
Bias (%) and precision (% coefficient of variation, CV) for High, Medium, and Low ranges were: Bias −1.02%, 4.14% and 6.08%; CV was 2.05%, 1.93%, 4.19%. Lower limit of detection was 0.15 μg.L⁻¹.

Disma N., Goffredo B.M., Cairoli S., Cirillo G., Morse J., Anderson B.J., Bonfiglio R., Cordani R., Garrone M., Patrone E., Iengo A., Bocca P., Izzo F., Diotto V., Lenares E., Robino C., Neri S., Colantonio L., Calderini E., Picardo S., Tucci I., Di Persio A., Montagnini L., Blesi L., Pistone B., Kuppers B., De Lorenzo B., Caramelli F, & Pasini L. (2022). Justification of empiric methodology to determine dexmedetomidine dose for the TREX study. Paediatric Anaesthesia, 33(3), 236-242.

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Quantitative Amino Acid Analysis in Biofluids

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Quantitative analysis of underivatized amino-acids in serum and urine samples was performed using the Amino Acids LC–MS/MS analysis Kit Jasem®. Liquid chromatography and mass spectrometry analysis was performed by a UHPLC Agilent 1290 Infinity II 6470 (Agilent Technologies) equipped with an ESI-JET-STREAM source operating in the positive ion (ESI+) mode. Equipment was controlled and data were analyzed using MassHunter Workstation (Agilent Technologies). The assay was validated for selectivity, specificity, linearity and limit of quantification, accuracy and precision, matrix effects and recovery, and stability.

Rega L.R., Janssens V., Graversen J.H., Moestrup S.K., Cairoli S., Goffredo B.M., Nevo N., Courtoy G.E., Jouret F., Antignac C., Emma F., Pierreux C.E, & Courtoy P.J. (2023). Dietary supplementation of cystinotic mice by lysine inhibits the megalin pathway and decreases kidney cystine content. Scientific Reports, 13, 17276.

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Quantitative Protein and Metabolite Analysis

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Dissected tissues were homogenized in 10 mM N-ethylmaleimide. Protein fraction was obtained following precipitation in 10% 5-sulfosalicylic acid and centrifugation at 20 000×g for 20 min at 4°C. Protein pellet was resuspended in 100 mM NaOH and assayed by the Bio-Rad Protein Assay according to the manufacturer’s instructions. Supernatant (50 μl) was mixed with 50 μl of the internal standard solution (Cystine d6) and vortexed for 5 s; then the mixture was extracted with 200 μl of acetonitrile, vortexed at least 30 s and then centrifuged at 13 000 rpm for 9 min. Liquid chromatography and mass spectrometry analysis was performed by a UHPLC Agilent 1290 Infinity II 6470 (Agilent Technologies) equipped with an ESI-JET-STREAM source operating in the positive ion (ESI+) mode. MassHunter Workstation (Agilent Technologies) software was used to control the equipment and analyze the data. The separation column was InfinityLab Poroshell 120 HILIC 1.9 μm 100 × 2.1 mm (Agilent Technologies). Validation of the method was performed based on the US Food and Drug Administration (FDA) guideline for industry bioanalytical method validation (FDA 2013) and European Medicines Agency (EMA) guideline (EMA 2011). The validation of the assay was performed including selectivity, specificity, linearity and limit of quantification, accuracy and precision, matrix effects and recovery, and stability.

De Leo E., Taranta A., Raso R., Polishchuk E., D’Oria V., Pezzullo M., Goffredo B.M., Cairoli S., Bellomo F., Battafarano G., Camassei F.D., Del Fattore A., Polishchuk R., Emma F, & Rega L.R. (2022). Genistein improves renal disease in a mouse model of nephropathic cystinosis: a comparison study with cysteamine. Human Molecular Genetics, 32(7), 1090-1101.

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