Western blotting was performed according to a standard method as previously described [20 (link)]. The antibodies used for immunoblotting were as follows: anti-CITED1, anti-cyclin B1, anti-cyclin D3, anti-cyclin A2, anti-cyclin D1, anti-cyclin E1, anti-CDK4, anti-CDK6 (Abcam, Cambridge, MA), anti-cyclin D2, anti-CDK2 (BD Pharmingen, San Diego, CA), anti-cyclin E2, anti-p21Cip1, anti-p27Kip1 (Cell Signaling Technology, Beverly, MA), and anti-α-tubulin (Sigma-Aldrich, St. Louis, Missouri). The bands were quantified using Quantity One software (Bio-Rad, Hercules, CA).
Anti cdk2
Manufactured by BD
Anti-CDK2 is a laboratory reagent used in scientific research. It functions as an inhibitor of the cyclin-dependent kinase 2 (CDK2) protein, which plays a critical role in cell cycle regulation. Anti-CDK2 can be used to study the mechanisms and effects of CDK2 inhibition in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti cyclin b1, by Abcam (3 mentions)
Anti cdk4, by Abcam (3 mentions)
Anti cyclin a2, by Abcam (3 mentions)
Anti cdk6, by Abcam (3 mentions)
Anti cyclin e1, by Abcam (3 mentions)
Anti cyclin d1, by Abcam (3 mentions)
Anti p21cip1, by Cell Signaling Technology (3 mentions)
Anti cyclin d3, by Abcam (3 mentions)
Anti p27kip1, by Cell Signaling Technology (3 mentions)
Anti cyclin e2, by Cell Signaling Technology (3 mentions)
Anti cyclin d2, by BD (3 mentions)
Anti α tubulin, by Merck Group (2 mentions)
Quantity one software, by Bio-Rad (1 mentions)
Mouse monoclonal anti α tubulin antibody, by Merck Group (1 mentions)
Anti rb, by Cell Signaling Technology (1 mentions)
Anti prb, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by BD (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Bicinchoninic acid assay, by Beyotime (1 mentions)
4 protocols using anti cdk2
1
Comprehensive Immunoblotting Analysis
2
Western Blot Analysis of Cell Cycle Regulators
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Western blotting analysis was performed according to a standard method previously described [31 (link)], using anti-SOSTDC1, anti-cyclin B1, anti-cyclin D3 (Abcam, Cambridge, MA), anti-cyclin A2, anti-cyclin D1, anti-cyclin E1, anti-CDK4, anti-CDK6 (Epitomics, Burlingame, California), anti-cyclin D2, anti-CDK2 (BD Pharmingen, San Diego, CA), anti-cyclin E2, anti-p21Cip1, anti-p27Kip1, anti-p-Rb Ser608, anti-p-Rb Ser807, anti-Rb (Cell Signaling, Beverly, MA). When re-probing, blotted membranes were stripped and re-blotted with an anti-α-tubulin mouse monoclonal antibody (Sigma–Aldrich, St. Louis, MO) as a loading control.
3
Western Blotting for Cell Cycle Proteins
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Western blotting was performed according to a standard method as described previously [32 (link)]. Antibodies for immunoblotting were as follows: anti-SOSTDC1, anti-cyclin B1, anti-cyclin D3 (Abcam, Cambridge, MA), anti-cyclin A2, anti-cyclin D1, anti-cyclin E1, anti-CDK4, anti-CDK6 (Epitomics, Burlingame, California), anti-cyclin D2, anti-CDK2 (BD Pharmingen, San Diego, CA), anti-cyclin E2, anti-p21Cip1, anti-p27Kip1, anti-p-pRb Ser608, anti-p-pRb Ser807, anti-pRb (Cell Signaling, Beverly, MA) and anti-α-tubulin (Sigma-Aldrich, St. Louis, Missouri).
4
Western Blot Analysis of MCF-7 Proteins
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Total cellular proteins from MCF-7 cells were extracted using RIPA lysis buffer (Beyotime Institute of Biotechnology). Protein concentration was determined using a bicinchoninic acid assay (Beyotime Institute of Biotechnology), and 10 µg protein/lane was separated by 10% SDS-PAGE followed by transferring to polyvinylidene fluoride membranes (EMD Millipore). The membranes were blocked with 5% non-fat milk for 1 h and incubated at 4°C with the following primary antibodies overnight: Anti-MYB (cat. no. ab191064; 1:1,000; BD Biosciences), anti-E-cadherin (cat. no. ab269767; 1:1,000; BD Biosciences), anti-N-cadherin (cat. no. ab18203; 1:1,000; BD Biosciences), anti-Bcl-2 (cat. no. ab194583; 1:1,000; BD Biosciences), anti-Bax (cat. no. ab104156; 1:1,000; BD Biosciences), anti-cyclin A1 (cat. no. ab53699; 1:1,000; BD Biosciences), anti-CDK2 (cat. no. ab64669; 1:1,000; BD Biosciences) and anti-GAPDH (cat. no. ab9485; 1:1,000; Affinity Biosciences). The membranes were washed three times using PBS, incubated with goat anti-rabbit IgG secondary antibody conjugated to horseradish peroxidase (cat. no. ab7090; 1:5,000; Abcam) for 1 h at room temperature and visualized using a Pierce ECL Western Blotting kit (Pierce; Thermo Fisher Scientific, Inc.). The protein expression was quantified using Image-Pro® Plus software (version 6.0; Media Cybernetics, Inc.).
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