Ovalbumin (OVA) was purchased from Sigma-Aldrich (USA). CpG oligonucleotide 1826 (5ʹ-TCC ATG ACG TTC CTG ACG TT-3ʹ) was synthesized by Sangon (China). Fetal bovine serum (FBS), phosphate-buffered saline (PBS), RPMI-1640, DMEM were purchased from Hyclone (USA). Rottlerin, chlorpromazine, Filipin III and cytochalasin D were purchased from ApexBio Technology (USA). Anhydrous dimethyl sulfoxide (DMSO), red blood cell lysis, the carbocyanine dye Dil, 4ʹ,6-Diamidino-2-phenylindole dihydrochloride (DAPI, ≥90%), fluorescein isothiocyanate (FITC) and near-Infrared Cyanine 7 dyes (Cy7 NHS ester) were purchased from Solarbio Science & Technology Co. Ltd (China). Cell Counting Kit-8 (CCK-8), NP-40 lysis buffer, X-Gal (ST912) and BCA protein detection kit were purchased from Beyotime (China). Recombinant mouse IL-4 and GM-CSF were purchased from Peprotech (USA). Anti-mouse monoclonal antibodies (CD80 (B7-1), CD86 (B7-2), CCR7, CD40, MHC I (H-2Kb) and MHC II (I-Ab)) were purchased from Biolegend (USA). None of the chemicals were further purified.
Rottlerin
Manufactured by Apexbio
Sourced in United States
Rottlerin is a chemical compound that is commonly used as a research tool in the field of cell biology and biochemistry. It is a natural product derived from the fruit of the plant Mallotus philippinensis. The primary function of Rottlerin is to inhibit the activity of the protein kinase C delta (PKC-δ) enzyme, which is involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant mouse il 4, by Thermo Fisher Scientific (1 mentions)
Filipin 3, by Apexbio (1 mentions)
Gm csf, by Thermo Fisher Scientific (1 mentions)
Cytochalasin d, by Apexbio (1 mentions)
Fluorescein isothiocyanate (fitc), by Solarbio (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Bca protein detection kit, by Beyotime (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Np 40 lysis buffer, by Beyotime (1 mentions)
Nocodazole, by Apexbio (1 mentions)
Wortmannin, by Apexbio (1 mentions)
Dynasore, by Apexbio (1 mentions)
Ammonium chloride nh4cl, by Merck Group (1 mentions)
Chlorpromazine cpz, by Selleck Chemicals (1 mentions)
Bafilomycin a1, by Apexbio (1 mentions)
Bafilomycin a, by Merck Group (1 mentions)
Methyl β cyclodextrin, by Merck Group (1 mentions)
2 protocols using rottlerin
1
Dendritic Cell Phenotype Characterization
2
Pharmacological Inhibition of Viral Endocytosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pharmacological inhibitors’ concentration used in our study is based on previous research (Table 1 ) [21 (link), 22 (link)]. Inhibitors were prepared as follows: pistop2, dynasore, rottlerin, nystatin, wortmannin, Bafilomycin A1, Latrunculin B, nocodazole, IPA-3, and amiloride were purchased from ApexBio (Houston, USA). Chlorpromazine (CPZ) was purchased from Selleck. Stock solutions of inhibitors were dissolved in dimethyl sulfoxide (DMSO), except Latrunculin B which was dissolved in ethanol according to the manufacturer’s instruction. Ammonium chloride (NH4Cl) and methyl-β-cyclodextrin were bought from Sigma and dissolved in H2O.
Variables from the questionnaire of the HBSC study analyzed in this study
| Inhibitor | Target | Highest concentration |
|---|---|---|
| Pistop2 | CME | 5 μM (GCRV 104); 25 μM (GCRV JX01) |
| CPZ | CME | 10 μM (GCRV 104; GCRV JX01) |
| Nystatin | Caveola-dependent endocytosis | 15 μM (GCRV 104) |
| methyl-β-cyclodextrin | Caveola-dependent endocytosis | 1 mM (GCRV 104) |
| dynasore | Dynamin | 10 μM (GCRV 104) |
| wortmannin | PI3K | 5 μM (GCRV 104) |
| IPA-3 | Pak1 | 20 μM (GCRV 104) |
| rottlerin | PKC | 10 μM (GCRV 104) |
| NH4Cl | PH | 20 mM (GCRV 104) |
| Amiloride | NHE | 10 μM (GCRV 104) |
| Bafilomycin A1 | Vacuolar ATPas inhibition | 2 nM (GCRV 104) |
| nocodazole | Microtubules | 10 μM (GCRV 104) |
| Latrunculin B | Actin microfilaments | 0.5 μM (GCRV 104) |
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!