Lipase

Lipase and zinc nitrate
hexahydrate were purchased from Thermo Fisher Scientific (U.K.). Disodium
monohydrogen phosphate, sodium dihydrogen phosphate, ethanol, curcumin,
ammonium tetrachloropalladate, dimethylamine borane (DMAB), methylene
blue (MB), and phenol were purchased from Sigma-Aldrich (U.K.). Graphene
oxide (GO) was synthesized in our lab (shown in the Supporting Information). All chemicals were used as received.
Kapok fibers and cotton fibers were picked in Nanning, China.

Primer-probes for the quantification of baseline RNA levels of TAP, CPA1, CPA2, amylase, lipase and two endogenous controls, PUM and GUS in tissue were purchased from Thermo Fisher Scientific (Waltham, MA, USA). TAP (Gene: Prss1; Assay: Rn00754931_m1; cat#4331182), CPA1 (Gene: CPA1; Assay: Rn00566512_m1; cat#4331182), CPA2 (Gene: CPA2, Assay: Rn01500585_m1; cat#4331182), amylase (Gene: Amy2a3; Assay: Rn00821330_g1; cat#4331182), pancreatic lipase (Gene: Pnlip; Assay: Rn00565851_m1; cat#4331182), PUM (Rn00982780_m1; cat# 4331182 ), GUSB (Rn00566655_m1; cat# 4331182). RNA from duodenum, liver and salivary gland was extracted using a Magmax liquid handler and the Magmax™-96 for Microarrays Total RNA Isolation Kit (all Thermo Fisher Scientific Waltham, MA, USA). Pancreas RNA was extracted with a trizol/chloroform/isopropanol precipitation method to avoid RNA degradation. Reverse transcription of 500 ng total RNA was performed in a 100 µL final volume with the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, cat # 4368814) on a Proflex instrument (Thermo Fisher Scientific). Quantitative PCR was performed in triplicate on a Quantstudio 12K Flex in a final volume of 10 µL with 5 µL of 2x Mastermix (Thermo Fisher Scientific cat#4324020), 0.5 µL of 20x Assay on demand (primer-probes) and 2.5 µL water.