Pe conjugated anti cd8 antibody

Manufactured by BD

Sourced in United States

The PE-conjugated anti-CD8 antibody is a reagent used in flow cytometry analysis. It binds specifically to the CD8 protein expressed on the surface of certain immune cells, allowing their identification and quantification.

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Lab products found in correlation

Fitc conjugated anti cd4 antibody, by BD (1 mentions) Cytoflex s, by Beckman Coulter (1 mentions) Red blood cell lysis buffer, by Solarbio (1 mentions) Negative selection kit, by Miltenyi Biotec (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Anti cd3 and anti cd28 antibodies, by BD (1 mentions)

Close spelling variants of this product

PE-conjugated anti-CD8 Anti-CD8 PE antibodies Anti-CD8-PE Anti-CD8 antibody CD8-PE CD8 antibody Anti-CD8

2 protocols using pe conjugated anti cd8 antibody

Quantifying CD4+ and CD8+ T Cells

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The blood samples (approximately 300 μL) were collected from mouse eyeball using a heparin sodium anticoagulant tube. The triple volume of red blood cell lysis buffer (Solarbio, Beijing, China) was added into the blood for 10 min and then centrifuged at 400 × g for 15 min. The supernatant was discarded, and the sedimentary leukomonocytes were resuspended with 300 μL PBS twice. Subsequently, 1 μL FITC-conjugated anti-CD4 antibody (BD, US) and 2 μL PE-conjugated anti-CD8 antibody (BD, US) were incubated with leukomonocytes for 30 min in a dark box. Then, leukomonocytes were rinsed with PBS 3 times. A total of 10,000 cells were counted for calculating the percentages of CD4 and CD8 positive cells using flow cytometry (CytoFLEX S, Beckman Coulter, US).

Liu X.B., Yu G.W., Gao X.Y., Huang J.L., Qin L.T., Ni H.B, & Lyu C. (2021). Intranasal delivery of plasmids expressing bovine herpesvirus 1 gB/gC/gD proteins by polyethyleneimine magnetic beads activates long-term immune responses in mice. Virology Journal, 18, 60.

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T Cell Cytotoxicity Assay for Breast Cancer

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CD4⁺ and CD8⁺ T cells were isolated from healthy donor PBMC using negative selection kits (Miltenyi Biotech). Cells were labeled with carboxyfluorescein succinimidyl ester (CFSE; Invitrogen) after which 3x10⁵ CD4⁺ or CD8⁺ T cells were combined with 1x10³ to 1x10⁴ breast cancer cells and seeded into 96-well plates. T cells were stimulated by the addition of anti-CD3 and anti-CD28 antibodies (3 μg/ml) (BD Biosciences). After 72 hours, cells were stained with allophycocyanin (APC)-conjugated anti-CD4 antibody, PE-conjugated anti-CD8 antibody and aqua live/dead stain (BD Biosciences). Flow cytometry analyses were performed as described above.

Mittendorf E.A., Philips A.V., Meric-Bernstam F., Qiao N., Wu Y., Harrington S., Su X., Wang Y., Gonzalez-Angulo A.M., Akcakanat A., Chawla A., Curran M., Hwu P., Sharma P., Litton J.K., Molldrem J.J, & Alatrash G. (2014). PD-L1 Expression in Triple Negative Breast Cancer. Cancer immunology research, 2(4), 361-370.

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