3flex adsorption analyzer

The samples of Mn-DHBQ were activated at 150 °C overnight under dynamic vacuum prior to single-component hydrocarbon vapor adsorption measurements. The single-component isotherms of C₆H₆, C₆H₁₀, and C₆H₁₂ were measured at 30, 60, 90 °C on a Micromeritics 3Flex adsorption analyzer equipped with a vapor dosing bottle. Each sample tube was subsequently immersed in a temperature-controlled heating mantle that surrounded most of the sample tube. The manifold of the instrument itself, including the vapor dosing bottle was heated to 25 °C and kept at this temperature for all these single-component C6 cyclic hydrocarbon measurements. The saturated pressure (P_sat) was defined as the vapor pressure of each hydrocarbon at 25 °C: C₆H₆ (12.7 kPa), C₆H₁₀ (12.0 kPa), C₆H₁₂ (13.0 kPa).

Nitrogen gas physisorption was conducted with Micromeritics 3Flex Adsorption Analyzer to study the porosity and hydrophobicity of the Pt/C and Pt/C-IL samples. The samples were first degassed at 150 °C for 12 h before analysis. Nitrogen physisorption was performed at 77 K between a relative pressure (P/P₀) of 0 and 0.995, wherein a low pressuring dosing (2 cm³ g⁻¹ STP) mode was used below 0.01 (P/P₀) for the microporous structure. The specific surface area was estimated by Brunauer–Emmett–Teller Model, and t-plot model was used to estimate the micropore volume/area and mesopore surface areas. The PSD above 2 nm was calculated with BJH model by using the adsorption isotherm. The PSD below 2 nm was estimated by non-linear DFT model assuming a slit pore geometry.

Powder
X-ray diffraction (PXRD) data were obtained at room temperature on
a Bruker D2 PHASER diffractometer using Cu-Kα radiation (λ
= 1.5418 Å), collecting in the 5–35° 2θ range,
with steps of 0.02° and a time per step of 0.5 s. The samples
were deposited in the hollow of a zero-background silicon sample holder.
Elemental analysis (EA) was performed in an elemental analyzer Thermo
Scientific FLASH 2000. ¹H and ³¹P nuclear magnetic
resonance spectroscopy (¹H- and ³¹P NMR) data
were obtained with a Bruker Nanobay AVANCE III HD 400 MHz (2-channel)
NMR spectrometer. Thermogravimetric analysis (TGA) was carried out
by a thermogravimetric analyzer Shimadzu mod. TGA-50H. Nitrogen adsorption
isotherms at 77 K were measured on a Micromeritics 3Flex adsorption
analyzer. Fourier transform infrared (FTIR) measurements were obtained
with a Bruker spectrophotometer. Scanning electron microscopy (SEM)
images were obtained on a Carl Zeiss SMT Auriga. Transmission electron
microscopy (TEM) images were obtained on a FEI Talos F200X microscope.
Microwave synthesis was carried out using an Anton Paar Monowave 300
reactor. DIFP adsorption experiments were performed in an Agilent
8860 GC chromatograph with an FID detector. The column used was HP-5
of 50 m length, 0.320 mm diameter, and 1.05 μm thickness. Enzymatic
assays were performed in a Tecan Infinite 200 PRO NanoQuant.

The physical and chemical properties of materials were analyzed with several methods. The surface morphologies and microchemical compositions of PA-1 and PA-2 were examined using a SEM/EDS (JSM-IT300), JEOL Ltd., Tokyo, Japan. The porosity and surface area (BET) were measured by the nitrogen adsorption isotherm at 77 K on a Micromeritics 3Flex adsorption analyzer, Micromeritics Instrument Corporation, USA. The surface functional group was measured using a FT-IR spectrometer, INVENIO, in a range of 400–4000 cm⁻¹, BRUKER, Germany. The points of zero charge (pH_PZC) of PA-1 and PA-2 were adapted from [33 (link)] and determined as follows: PA-1 and PA-2 were weighed for 1.0 g and put in 200 mL of deionized water at a pH range of 1.0 to 14.0, adjusted by nitric acid or sodium hydroxide. Then, the mixed samples were shaken by using a shaker (GFL, Orbital shaker 3017, Greater Hanover, Germany) at 200 rpm for 24 h. After that, final pH of each sample was measured. The initial and final pH values were plotted to obtain the point of zero charge (pH_PZC) at the crossing point between the lines connecting the pH data and the diagonals connecting the equal initial and final pH.

Ti-2448 alloys were provided by the Institute of Metal Research, Chinese Academy of Sciences (Shenyang, China). Disks (diameter: 10 mm, thickness: 1 mm) were used for the cell culture experiments. Cylindrical implants (diameter: 2 mm, length: 6 mm) were used for endosseous implantation. Ti-2448 alloys were regarded as a negative control and substrate. All samples were ground with a 1200-grit SiC sandpaper and cleaned ultrasonically in acetone, ethanol, and deionized water, successively. Finally, a ZSM-5 coating was synthesized on the surface of the alloy through the hydrothermal method [10 (link)]. The materials were ultrasonically cleaned, autoclaved (134°C/0.21 MPa), and prepared for use. The surface morphologies of the samples were observed by scanning electron microscopy (SEM, Hitachi S3400N, Japan) and energy-dispersive spectrometry (EDS, Inca, Oxford). The sample surface wettability was analyzed using a contact angle measurement system (VSA 2500 XE, AST Products, Tokyo, Japan). Infrared spectra of the prepared samples were measured using a Fourier-transform infrared (FT-IR) spectroscopy (Thermo Fisher Scientific Nicolet 6700, Madison, WI, USA). The texture properties were evaluated by the nitrogen adsorption/desorption method at 77 K using a Micromeritics 3Flex Adsorption Analyzer (Micromeritics, USA).