α ketoglutarate colorimetric fluorometric assay kit

Manufactured by Abcam

Sourced in United States

The α-Ketoglutarate Colorimetric/Fluorometric Assay Kit is a laboratory tool used to quantify the levels of α-ketoglutarate, a key intermediate in the citric acid cycle. The kit provides a simple, sensitive, and accurate method for measuring α-ketoglutarate concentrations in various biological samples.

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Lab products found in correlation

Glutamine assay kit, by Abnova (1 mentions) Atp assay kit, by Beyotime (1 mentions) Acetyl coa assay kit, by Merck Group (1 mentions) Citrate assay kit, by Merck Group (1 mentions) Free fatty acid quantification assay kit, by Abcam (1 mentions) Succinate succinic acid colorimetric assay kit, by Abcam (1 mentions)

4 protocols using α ketoglutarate colorimetric fluorometric assay kit

Determination of Glycolate, Glyoxylate, and 2-OG

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Glycollate and glyoxylate were determined according to Xu et al. [28 (link)] and the content of 2-OG were measured using α-Ketoglutarate Colorimetric/Fluorometric Assay Kit (BioVision, Catalog #K677, Milpitas, USA) according to the manufacturer’s instructions.

Lin Y., Zhang J., Gao W., Chen Y., Li H., Lawlor D.W., Paul M.J, & Pan W. (2017). Exogenous trehalose improves growth under limiting nitrogen through upregulation of nitrogen metabolism. BMC Plant Biology, 17, 247.

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Cellular Metabolite Quantification

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Cells were seeded in 6-well plates for overnight. After the indicated treatments for 72h, the concentrations of glutamine, ATP or α-KG was measured by Glutamine Assay Kit (Abnova, KA1627), ATP Assay Kit (Beyotime, S0026) or α-Ketoglutarate Colorimetric/Fluorometric Assay Kit (BioVision, K677-100), based on the protocols provided in the kit.

Li J., Song P., Zhu L., Aziz N., Zhou Q., Zhang Y., Xu W., Feng L., Chen D., Wang X, & Jin H. (2017). Synthetic lethality of glutaminolysis inhibition, autophagy inactivation and asparagine depletion in colon cancer. Oncotarget, 8(26), 42664-42672.

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Metabolic Profiling of Activated Naive T Cells

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CD4⁺CD45RA⁺ naive T cells were stimulated with anti-CD3/anti-CD28-coated beads and cultured for 72 h. α-Ketoglutarate Color-imetric/Fluorometric Assay Kit (BioVision), Succinate Assay Kit (BioAssay Systems), Citrate Assay Kit (Sigma-Aldrich), Acetyl-CoA Assay Kit (Sigma-Aldrich), Free Fatty Acid Quantification Assay Kit (Abcam) were used to measure intracellular α-ketoglutarate, succinate, citrate, acetyl-CoA and free fatty acids.

Wu B., Qiu J., Zhao T.V., Wang Y., Maeda T., Goronzy I.N., Akiyama M., Ohtsuki S., Jin K., Tian L., Goronzy J.J, & Weyand C.M. (2020). Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells. Cell metabolism, 32(6), 967-980.e5.

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Metabolite Quantification in Macrophages

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α-ketoglutarate and succinate levels in macrophages were measured by α-Ketoglutarate Colorimetric/Fluorometric Assay Kit (BioVision, Cat#K677) and Succinate (Succinic Acid) Colorimetric Assay Kit (BioVision, Cat#K649) respectively as previously described^{25 (link)}. Glutaminase activity were measured using PicoProbe™ Glutaminase (GLS) Activity Assay Kit (Fluorometric) (BioVision, Cat#K455) according the manual as previously described⁵³.

Zhou B., Magana L., Hong Z., Huang L.S., Chakraborty S., Tsukasaki Y., Huang C., Wang L., Di A., Ganesh B., Gao X., Rehman J, & Malik A.B. (2020). The angiocrine Rspondin3 instructs interstitial macrophage transition via metabolic-epigenetic reprogramming and resolves inflammatory injury. Nature immunology, 21(11), 1430-1443.

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