BUN was measured using the urease indophenol method (Wako Pure Chemical Industries). An absorbance 96-well plate reader (Spectra MAX Plus; Molecular Devices, Sunnyvale, CA, USA) was used at a wavelength of 570 nm. The concentrations of IL-1β, IL-2, IL-6, TNF-α, and IL-10 were determined in the plasma, using custom V-plex assays (Meso scale discovery, Rockville, MD, USA) according to the manufacturer’s protocol. The plasma HMGB1 concentration was measured using an established enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (Fuso pharmaceutical, Osaka, Japan).
Urease indophenol method
Manufactured by Fujifilm
Sourced in Japan
The Urease indophenol method is a lab equipment product that is used to measure urea levels in biological samples. It utilizes the enzymatic conversion of urea to ammonia, which is then detected colorimetrically using the indophenol reaction. This method provides a quantitative assessment of urea concentration in the sample.
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Lab products found in correlation
2 protocols using urease indophenol method
1
Measuring Inflammatory Markers in Plasma
2
Serum urea and creatinine analysis
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Serum concentrations of urea nitrogen were determined by the urease‐indophenol method (Wako Pure Chemical, Osaka, Japan). Serum creatinine concentrations were measured by an enzymatic method (Wako Pure Chemical).
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